Shandong Provincial Key Laboratory of Detection Technology, for Tumour Markers, College of Chemistry and Chemical Engineering, Linyi University, Linyi, 276005, P. R. China.
Shandong Sino-Japanese Center for Collaborative Research, of Carbon Nanomaterials, College of Chemistry and Chemical Engineering, Qingdao University, Shandong, 266071, P. R. China.
Chemistry. 2018 Apr 25;24(24):6458-6463. doi: 10.1002/chem.201800154. Epub 2018 Apr 14.
Intracellular [Ca ] and pH have a close relationship, and their abnormal levels can result in cell dysfunction and accompanying diseases. Thus, simultaneous determination of [Ca ] and pH can more accurately investigate complex biological processes in an integrated platform. Herein, multicolor upconversion nanoparticles (UCNPs) were prepared with the advantages of no spectral overlapping, single NIR excitation wavelengths, and greater tissue penetration depth. The upconversion nanoprobes were easily prepared by the attachment of two fluorescent dyes, Fluo-4 and SNARF-4F. Based on the dual luminescence resonance energy transfer (LRET) process, the blue and green fluorescence of the UCNPs were specially quenched and selectively recovered after the detachment and/or absorbance change of the attached fluorescent dyes, enabling dual detection. Importantly, the developed nanoprobe could successfully be applied for the detection of [Ca ] and pH change in adenosine triphosphate (ATP) and ethylene glycol tetraacetic acid (EGTA) stimulation in living cells.
细胞内的[Ca2+]和 pH 值密切相关，其异常水平会导致细胞功能障碍和相关疾病。因此，同时测定[Ca2+]和 pH 值可以更准确地在一个综合平台上研究复杂的生物过程。本文采用上转换纳米粒子（UCNPs）作为荧光探针，其具有无光谱重叠、单一近红外激发波长和更大的组织穿透深度等优点。上转换纳米探针是通过附着两种荧光染料 Fluo-4 和 SNARF-4F 制备的。基于双荧光共振能量转移（LRET）过程，在附着的荧光染料的分离和/或吸收变化后，UCNPs 的蓝色和绿色荧光会被特异性猝灭和选择性恢复，从而实现双重检测。重要的是，该纳米探针可成功应用于活细胞中三磷酸腺苷（ATP）和乙二胺四乙酸（EGTA）刺激下[Ca2+]和 pH 值变化的检测。
