Lady Davis Institute for Medical Research, Jewish General Hospital, Montreal, QC, Canada.
Department of Dentistry, Jewish General Hospital, Montreal, QC, Canada.
Mov Disord. 2018 Apr;33(4):583-591. doi: 10.1002/mds.27328. Epub 2018 Feb 28.
To date, there are no chemical analytes, including biochemical indices of oxidative stress, metabolites of α-synuclein protein, and differential protein expression patterns on proteomic profiling, for use in clinics as a diagnostic biomarker of idiopathic PD.
Heme oxygenase-1 has been implicated in the pathogenesis of PD. The objective of this study is to ascertain whether salivary heme oxygenase-1 may serve as a biomarker for early idiopathic PD.
Fifty-eight PD patients and 59 non-neurological disease controls were recruited. Levels of heme oxygenase-1 expression were assayed using enzyme-linked immunosorbent assay and western blot analysis of whole, unstimulated saliva. Analyses were adjusted by sex, l-dopa exposure, and relevant comorbidities.
We documented: (1) the presence of 32-kDa heme oxygenase-1 protein in human saliva; (2) significantly higher mean heme oxygenase-1 protein concentrations in saliva of PD patients relative to control values; (3) no variability in salivary heme oxygenase-1 levels with sex, age, l-dopa equivalence, or comorbidities; and (4) significantly higher mean salivary heme oxygenase-1 concentrations in patients with H & Y stage 1 PD (early) than control subjects and stage 2 and stage 3 PD patients. The area under the receiver operating characteristic curve that separated controls from PD H & Y stage 1 was 76% (95% confidence interval: 63-90).
Salivary heme oxygenase-1 concentrations may provide a useful, noninvasive, and relatively inexpensive biomarker of early idiopathic PD. © 2018 International Parkinson and Movement Disorder Society.
迄今为止，还没有任何化学分析物（包括氧化应激的生化指标、α-突触核蛋白的代谢物和蛋白质组学分析中的差异蛋白表达模式）可用于临床作为特发性 PD 的诊断生物标志物。
血红素加氧酶-1 已被牵涉到 PD 的发病机制中。本研究的目的是确定唾液血红素加氧酶-1 是否可作为早期特发性 PD 的生物标志物。
招募了 58 名 PD 患者和 59 名非神经疾病对照者。使用酶联免疫吸附试验和全唾液非刺激物的 Western blot 分析检测血红素加氧酶-1 的表达水平。通过性别、l-多巴暴露和相关合并症进行分析调整。
我们记录到：（1）人唾液中存在 32-kDa 血红素加氧酶-1 蛋白；（2）与对照值相比，PD 患者的唾液血红素加氧酶-1 蛋白浓度明显更高；（3）唾液血红素加氧酶-1 水平不受性别、年龄、l-多巴等效物或合并症的影响；（4）H & Y 分期 1（早期）的 PD 患者的平均唾液血红素加氧酶-1 浓度明显高于对照者和 H & Y 分期 2 和 3 的 PD 患者。将对照者与 PD H & Y 分期 1 区分开的接收者操作特征曲线下面积为 76%（95%置信区间：63-90）。
唾液血红素加氧酶-1 浓度可能为早期特发性 PD 提供一个有用的、非侵入性的和相对廉价的生物标志物。 © 2018 国际帕金森和运动障碍学会。
