Department of Clinical Laboratory, The Fourth Affiliated Hospital of Harbin Medical University, Harbin, Heilongjiang 150001, P.R. China.
Department of Clinical Biochemistry, Chinese PLA General Hospital, Beijing 100853, P.R. China.
Mol Med Rep. 2018 May;17(5):6261-6268. doi: 10.3892/mmr.2018.8643. Epub 2018 Feb 27.
An increase in serum homocysteine level has been associated with an increased risk of vascular disease; however, the biochemical mechanisms that underlie these effects remain largely unknown. The present study aimed to use high-performance liquid chromatography-mass spectrometry (HPLC‑MS) to demonstrate the effects of serum homocysteine on human blood metabolites. A total of 75 fasting serum samples were investigated in the present study. Using a threshold of 15 µmol/l serum homocysteine level, samples were divided into high‑ and low‑homocysteine groups, and the serum extracts were analyzed with an HPLC‑MS‑based method. A total of 269 features exhibited significant differences and correlation with serum homocysteine levels in the electrospray ionization‑positive [ESI(+)] mode, and 69 features were identified in the ESI(‑) mode between the two groups. The principal component analysis plot revealed a separation between the high‑ and the low‑homocysteine groups. Metabolite set enrichment analysis identified arachidonic acid metabolism and linoleic acid metabolism as the two pathways with significantly enriched differences. These results revealed that arachidonic acid and linoleic acid metabolism may be associated with serum homocysteine levels and may be involved in homocysteine-induced vascular disease.
血清同型半胱氨酸水平升高与血管疾病风险增加有关；然而，这些影响背后的生化机制在很大程度上仍不清楚。本研究旨在使用高效液相色谱-质谱法（HPLC-MS）来证明血清同型半胱氨酸对人体血液代谢物的影响。本研究共调查了 75 例空腹血清样本。使用 15 μmol/l 血清同型半胱氨酸水平的阈值，将样本分为高同型半胱氨酸组和低同型半胱氨酸组，并使用基于 HPLC-MS 的方法分析血清提取物。在正电喷雾电离（ESI(+）模式下，共 269 个特征与血清同型半胱氨酸水平有显著差异和相关性，在 ESI(-)模式下，两组之间有 69 个特征。主成分分析图显示了高同型半胱氨酸组和低同型半胱氨酸组之间的分离。代谢物集富集分析确定花生四烯酸代谢和亚油酸代谢为两个具有显著富集差异的途径。这些结果表明，花生四烯酸和亚油酸代谢可能与血清同型半胱氨酸水平相关，并可能参与同型半胱氨酸诱导的血管疾病。
