Politi L, Adler R
Invest Ophthalmol Vis Sci. 1987 Jan;28(1):118-25.
This article reports that cultured photoreceptor cells are selectively vulnerable to complement-mediated lysis by antiopsin antisera. The study has been carried out using a culture system that permits the growth of embryonic retinal neurons and photoreceptor cells in the absence of glial, pigment epithelial, connective, or endothelial cell contamination. Exposure of these cultures to an antiopsin antiserum in the presence of complement results in the lysis of 85% of the photoreceptor cells, without extensive loss of neurons. Photoreceptor lysis by the antibody is dose-dependent and specific, as demonstrated by the ability to block photoreceptor lysis by preincubation of the antiopsin antiserum with purified rhodopsin. Photoreceptor sensitivity to lysis by these antibodies develops in vitro in parallel with the appearance of immunocytochemically detectable opsin. Multipolar neurons (which do not contain opsin) are not affected by the treatment, as shown by microscopic analysis and determination of neuronal "markers" such as choline acetyltransferase and GABA high affinity uptake. These results show that antiopsin antibodies can cause selective photoreceptor degeneration. Moreover, this in vitro system appears useful as a bioassay to test a variety of possible mechanisms of retinal cell destruction which may be important in vivo. The results suggest also a mechanism for the generation of enriched retinal neuronal cultures by selective lysis of photoreceptor cells.
本文报道，培养的光感受器细胞对抗视蛋白抗血清介导的补体溶解具有选择性易损性。该研究使用了一种培养系统，该系统可使胚胎视网膜神经元和光感受器细胞在无神经胶质细胞、色素上皮细胞、结缔组织细胞或内皮细胞污染的情况下生长。在补体存在的情况下，将这些培养物暴露于抗视蛋白抗血清会导致85%的光感受器细胞溶解，而神经元不会大量损失。抗体介导的光感受器溶解具有剂量依赖性和特异性，这通过将抗视蛋白抗血清与纯化的视紫红质预孵育来阻断光感受器溶解的能力得以证明。这些抗体介导的光感受器对溶解的敏感性在体外与免疫细胞化学可检测到的视蛋白的出现同时发展。多极神经元（不含视蛋白）不受该处理的影响，显微镜分析以及对神经元“标志物”如胆碱乙酰转移酶和GABA高亲和力摄取的测定均表明了这一点。这些结果表明，抗视蛋白抗体可导致选择性光感受器变性。此外，这种体外系统似乎可用作生物测定法，以测试多种可能在体内很重要的视网膜细胞破坏机制。研究结果还提示了一种通过选择性溶解光感受器细胞来生成富集视网膜神经元培养物的机制。
