Cândido-Soares Lara E, Martinez Elizabeth F, de Araújo Vera C, Araújo Ney S, Freitas Nadir S, Napimoga Marcelo H
Laboratory of Oral Pathology.
Laboratory of Immunology and Molecular Biology, São Leopoldo Mandic Institute and Research Center, Campinas, Sa[Combining Tilde]o Paulo, Brazil.
Appl Immunohistochem Mol Morphol. 2019 Jul;27(6):448-453. doi: 10.1097/PAI.0000000000000648.
Secreted osteoclastogenic factor of activated T cells (SOFAT) is a novel activated human T-cell-secreted cytokine that induce osteoclastogenesis in a RANKL-independent manner. The aim of this study was to evaluate the immunohistochemical expression of SOFAT in intraosseous and extraosseous lesions. Thirty-two oral biopsies were divided into 2 groups: (1) intraosseous lesions-4 cases of cherubism, 5 central giant cell lesions, 3 osteoblastomas, 3 cementoblastomas, 2 periapical lesions and (2) extraosseous lesions-5 peripheral giant cell lesions, 5 cases of oral paracoccidioidomycosis, and 5 foreign body reactions. Immunohistochemistry was performed for SOFAT and tartrate-resistant acid phosphatase. Image analysis consisted of a descriptive evaluation of the immunohistochemical staining pattern observed. Tartrate-resistant acid phosphatase-positive lesions included those containing multinucleated giant cells (MGC) from both groups. SOFAT was positive in MGC of the intraosseous lesions group, except in periapical foreign body reactions as well as extraosseous lesions. SOFAT was shown to be a putative marker of osteoclasts, which proved useful to differentiate them from multinucleated macrophages. Osteoclast induction may be both dependent and independent from the RANK/RANKL/OPG pathway and independent from the bone microenvironment.
活化T细胞分泌的破骨细胞生成因子（SOFAT）是一种新型的活化人T细胞分泌的细胞因子，它以不依赖RANKL的方式诱导破骨细胞生成。本研究的目的是评估SOFAT在骨内和骨外病变中的免疫组化表达。32例口腔活检标本分为2组：（1）骨内病变组——4例 cherubism、5例中央巨细胞病变、3例成骨细胞瘤、3例成牙骨质细胞瘤、2例根尖周病变；（2）骨外病变组——5例外周巨细胞病变、5例口腔副球孢子菌病和5例异物反应。对SOFAT和抗酒石酸酸性磷酸酶进行免疫组化检测。图像分析包括对观察到的免疫组化染色模式进行描述性评估。抗酒石酸酸性磷酸酶阳性病变包括两组中含有多核巨细胞（MGC）的病变。除根尖周异物反应和骨外病变外，骨内病变组的MGC中SOFAT呈阳性。SOFAT被证明是破骨细胞的一个假定标志物，有助于将其与多核巨噬细胞区分开来。破骨细胞诱导可能既依赖也不依赖RANK/RANKL/OPG途径，且不依赖于骨微环境。
