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用于超分辨率成像的编码孔径结构光照明显微术

Coded aperture structured illumination digital holographic microscopy for superresolution imaging.

作者信息

Lai Xin-Ji, Tu Han-Yen, Lin Yu-Chih, Cheng Chau-Jern

出版信息

Opt Lett. 2018 Mar 1;43(5):1143-1146. doi: 10.1364/OL.43.001143.

DOI:10.1364/OL.43.001143
PMID:29489800
Abstract

This paper proposes a coded aperture structured illumination (CASI) technique in digital holographic microscopy (DHM). A CASI wave is generated using two binary phase codes (0° and 120°) for spatial phase shifting. The generated CASI wave then interferes with a reference wave to form a coded Fresnel hologram at a single exposure with compressive sensing (CS) to avoid the temporal phase-shifting process of the structured illumination (SI). The CS algorithm is applied to retrieve the missing data of decoded phase-shifted SI-modulated waves, which are used to separate overlapped spatial frequencies for obtaining a larger spatial frequency coverage to provide superresolution imaging. Two phase-only spatial light modulators are applied to generate a directional SI pattern for obtaining a coded aperture with a suitable size to perform one-shot acquisition in the DHM system.

摘要

本文提出了一种数字全息显微镜(DHM)中的编码孔径结构照明(CASI)技术。使用两个二进制相位编码(0°和120°)生成CASI波用于空间相移。然后,生成的CASI波与参考波干涉,在单次曝光时通过压缩感知(CS)形成编码菲涅耳全息图,以避免结构照明(SI)的时间相移过程。应用CS算法检索解码后的相移SI调制波的缺失数据,这些数据用于分离重叠的空间频率,以获得更大的空间频率覆盖范围,从而提供超分辨率成像。应用两个纯相位空间光调制器生成定向SI图案,以获得具有合适尺寸的编码孔径,以便在DHM系统中进行单次采集。

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