Institute of Electro-Optical Science and Technology, National Taiwan Normal University, Taipei, 11677, Taiwan.
Department of Electrical Engineering, Chinese Culture University, Taipei, 11114, Taiwan.
Sci Rep. 2019 Jul 19;9(1):10489. doi: 10.1038/s41598-019-46951-w.
In this study, a novel adaptive wavefront correction (AWC) technique is implemented on a compactly developed structured illumination holographic tomography (SI-HT) system. We propose a mechanical movement-free compact scanning architecture for SI-HT systems with AWC, implemented by designing and displaying a series of computer-generated holograms (CGH) composed of blazed grating with phase Fresnel lens on a phase-only spatial light modulator (SLM). In the proposed SI-HT, the aberrations of the optical system are sensed by digital holography and are used to design the CGH-based AWC to compensate the phase aberrations of the tomographic imaging system. The proposed method was validated using a standard Siemens star target, its potential application was demonstrated using a live candida rugosa sample, and its label-free three-dimensional refractive index profile was generated at its subcellular level. The experimental results obtained reveal the ability of the proposed method to enhance the imaging performance in both lateral and axial directions.
本研究在一个紧凑的结构光照明显微层析（SI-HT）系统上实现了一种新颖的自适应波前校正（AWC）技术。我们提出了一种具有 AWC 的机械无运动紧凑扫描架构，通过在相位光调制器（SLM）上设计和显示由相位菲涅尔透镜组成的闪耀光栅的一系列计算机生成全息图（CGH）来实现。在所提出的 SI-HT 中，光学系统的像差通过数字全息术来感知，并用于设计基于 CGH 的 AWC 来补偿层析成像系统的相位像差。使用标准的西门子星靶对提出的方法进行了验证，使用活的 Rugosa 假丝酵母样本证明了其潜在应用，并在亚细胞水平生成了其无标记的三维折射率分布。实验结果表明，该方法能够增强横向和轴向的成像性能。
