Department of Cell Biology and Genetics, Faculty of Science, Palacky University, Olomouc, Czech Republic.
Department of Pathophysiology and Allergy Research, Medical University of Vienna, Vienna, Austria.
PLoS One. 2018 Feb 28;13(2):e0193655. doi: 10.1371/journal.pone.0193655. eCollection 2018.
The role of vitamin D receptor (VDR) in immune responses has been broadly studied and it has been shown that activated VDR alters the levels of some interleukins (ILs). In this study, we studied the opposite, i.e. whether 13 selected pro-inflammatory and anti-inflammatory ILs influence the transcriptional activity of human VDR. The experimental models of choice were two human stably transfected gene reporter cell lines IZ-VDRE and IZ-CYP24, which were designed to evaluate the transcriptional activity of VDR. The gene reporter assays revealed inhibition of calcitriol-induced luciferase activity by IL-4 and IL-13, when 1 ng/mL of these two compounds decreased the effect of calcitriol down to 60% of the control value. Consistently, calcitriol-induced expression of CYP24A1 mRNA was also significantly decreased by IL-4 and IL-13. The expression of VDR and CYP27B1 mRNAs was not influenced by any of the 13 tested ILs. These data suggest possible cross-talk between the VDR signalling pathway and IL-4- and IL-13-mediated cell signalling.
维生素 D 受体 (VDR) 在免疫反应中的作用已被广泛研究，研究表明激活的 VDR 会改变某些白细胞介素 (IL) 的水平。在这项研究中，我们研究了相反的情况，即 13 种选定的促炎和抗炎白细胞介素是否会影响人 VDR 的转录活性。选择的实验模型是两个稳定转染基因报告细胞系 IZ-VDRE 和 IZ-CYP24，旨在评估 VDR 的转录活性。基因报告测定显示，白细胞介素 4 和白细胞介素 13 抑制了钙三醇诱导的荧光素酶活性，当这两种化合物的浓度为 1ng/ml 时，钙三醇的作用降低到对照值的 60%。一致地，白细胞介素 4 和白细胞介素 13 也显著降低了钙三醇诱导的 CYP24A1 mRNA 的表达。VDR 和 CYP27B1 mRNA 的表达不受 13 种测试的白细胞介素中的任何一种的影响。这些数据表明 VDR 信号通路与白细胞介素 4 和白细胞介素 13 介导的细胞信号之间可能存在交叉对话。
