来自植物的病毒双链RNA（dsRNA）：用于高通量测序的替代性核酸底物
Viral Double-Stranded RNAs (dsRNAs) from Plants: Alternative Nucleic Acid Substrates for High-Throughput Sequencing.
作者信息
Marais Armelle, Faure Chantal, Bergey Bernard, Candresse Thierry
机构信息
UMR 1332, Biologie du Fruit et Pathologie, INRA, Univ. Bordeaux, Villenave d'Ornon, France.
出版信息
Methods Mol Biol. 2018;1746:45-53. doi: 10.1007/978-1-4939-7683-6_4.
High-throughput sequencing (or next-generation sequencing-NGS) is an emerging technology that allows the detection of plant viruses without any prior knowledge. Various sequencing techniques and various templates can be used as substrate for NGS. This chapter describes an optimized protocol for the extraction of double-stranded RNAs (dsRNAs) from a wide range of plants and for their random amplification prior to NGS sequencing.
高通量测序（或新一代测序 - NGS）是一种新兴技术，可在无需任何先验知识的情况下检测植物病毒。各种测序技术和各种模板都可作为NGS的底物。本章介绍了一种优化方案，用于从多种植物中提取双链RNA（dsRNA）并在NGS测序之前对其进行随机扩增。
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