Co-Innovation Center for Sustainable Forestry in Southern China, Key Laboratory of Forest Genetics and Biotechnology, Ministry of Education, Nanjing Forestry University, Nanjing 210037, China.
Australian Research Council Center of Excellence in Plant Energy Biology, School of Agriculture, Food and Wine, University of Adelaide, Glen Osmond, SA 5064, Australia.
Plant Physiol Biochem. 2018 May;126:22-31. doi: 10.1016/j.plaphy.2018.01.023. Epub 2018 Feb 21.
Drought and salinity are two main abiotic stressors that can disrupt plant growth and survival. Various biotechnological approaches have been used to alleviate the problem of drought stress by improving water stress resistance in forestry and agriculture. The drought sensitive 1 (DRS1) gene acts as a regulator of drought stress, identified in human, yeast and some model plants, such as Arabidopsis thaliana, but there have been no reports of DRS1 transformation in poplar plants to date. In this study, we transformed the DRS1 gene from Populus trichocarpa into Populus deltoides × Populus euramericana 'Nanlin895' using Agrobacterium tumefaciens-mediated transformation. We confirmed that the DRS1 gene was transformed into 'Nanlin895' poplar genomes using reverse transcription polymerase chain reaction (PCR), multiplex PCR, real-time PCR, and sodium dodecyl sulfate-polyacrylamide gel electrophoresis. All transformed and wild-type (WT) plants were then transferred into a greenhouse for complementary experiments. We analyzed the physiological and biochemical responses of transgenic plants under drought and salt stresses in the greenhouse, and the results were compared with control WT plants. Responses to abiotic stress were greater in transgenic plants compared with WT. Based on our results, introduction of the DRS1 gene into poplar 'Nanlin895' plants significantly enhanced the resistance of those plants to water deficit and high salinity, allowing higher growth rates of roots and shoots in those plants. Additionally, the clawed root rate increased in transformed poplars grown in culture media or in soil, and improved survival under drought and salt stress conditions.
干旱和盐度是两种主要的非生物胁迫因子，它们会破坏植物的生长和存活。各种生物技术方法已被用于通过提高林业和农业的耐水胁迫能力来缓解干旱胁迫问题。在人类、酵母和一些模式植物（如拟南芥）中鉴定到的干旱敏感 1 (DRS1) 基因作为干旱胁迫的调节剂，但迄今为止，尚未有关于将 DRS1 基因转化为杨树的报道。在这项研究中，我们使用根癌农杆菌介导的转化法，将毛果杨中的 DRS1 基因转化到杂种欧美杨×山杨 '南林 895' 中。我们通过反转录聚合酶链反应 (PCR)、多重 PCR、实时 PCR 和十二烷基硫酸钠-聚丙烯酰胺凝胶电泳，证实 DRS1 基因已转化到 '南林 895' 杨的基因组中。然后，所有转化和野生型 (WT) 植株均被转移到温室中进行互补实验。我们在温室中分析了干旱和盐胁迫下转基因植株的生理和生化反应，并将结果与对照 WT 植株进行了比较。与 WT 相比，转基因植株对非生物胁迫的反应更大。根据我们的结果，将 DRS1 基因导入到 'Nanlin895' 杨中，显著提高了这些植株对水分亏缺和高盐度的抗性，使这些植株的根和茎生长速度更高。此外，在培养介质或土壤中生长的转基因杨树的爪状根率增加，并提高了在干旱和盐胁迫条件下的存活率。
