Duan L X, Li X L, Hu P W, Luo R, Luo X, Chen Y Y
Department of Epidemiology and Health Statistics, Xiangya School of Public Health, Central South University, Changsha 410078, China.
Department of Scientific Research, Xiangya Hospital, Central South University, Changsha 410008, China.
Zhonghua Liu Xing Bing Xue Za Zhi. 2018 Feb 10;39(2):194-198. doi: 10.3760/cma.j.issn.0254-6450.2018.02.011.
To investigate the association between three single nucleotide polymorphism (SNP) genes DRD2 (rs1800497, rs6275, and rs1799978) and the dosage used on methadone maintenance treatment (MMT). From the methadone maintenance treatment centers, 257 MMT patients were recruited to participate in a case-control study and divided into two groups-control groups under low dosage (=89) and case (=168) group with high dosage. Quanto software was used to estimate the sample size as 180. Information related to social-demographic status, history on drug use and medication were collected. And DRD2 SNPs were genotyped to explore the relationship between polymorphism of DRD2 gene and the dosage of methadone maintenance treatment. Distributions of DRD2 rs6275 between different groups were significantly different. Patients carrying TC genotype needed lower dose of methadone when compared to the patients that carrying CC genotype counterparts (=0.338, 95% : 0.115-0.986). Patients that carrying C allele at rs6275 needed lower methadone dose than those that carrying genotype TT (=0.352, 95% : 0.127-0.975). Distributions of genotypes, alles in the other two SNPs (rs1800497, rs1799978) were not significantly different between groups under different dosages. DRD2 rs6275 was associated with dosage of methadone used for the MMT patients. However, no significant associations were found between rs1800497, rs1799978 and the dosage of methadone.
为研究三个单核苷酸多态性（SNP）基因DRD2（rs1800497、rs6275和rs1799978）与美沙酮维持治疗（MMT）用药剂量之间的关联。从美沙酮维持治疗中心招募了257名MMT患者参与一项病例对照研究，并分为两组——低剂量对照组（=89）和高剂量病例组（=168）。使用Quanto软件估计样本量为180。收集了与社会人口学状况、吸毒史和用药情况相关的信息。对DRD2单核苷酸多态性进行基因分型，以探讨DRD2基因多态性与美沙酮维持治疗剂量之间的关系。不同组之间DRD2 rs6275的分布存在显著差异。与携带CC基因型的患者相比，携带TC基因型的患者需要的美沙酮剂量更低（=0.338，95%置信区间：0.115 - 0.986）。在rs6275位点携带C等位基因的患者比携带TT基因型的患者需要更低的美沙酮剂量（=0.352，95%置信区间：0.127 - 0.975）。在不同剂量组之间，其他两个SNP（rs1800497、rs1799978）的基因型和等位基因分布没有显著差异。DRD2 rs6275与MMT患者使用的美沙酮剂量相关。然而，未发现rs1800497、rs1799978与美沙酮剂量之间存在显著关联。
