Wang S Y, Cui S, Li Z Q, Ji L H, Ma J, Liu H H, Zhang G Y, Suo S H, Ge R L
Medical College of Qinghai University, Xining 810001, China.
Zhonghua Yi Xue Za Zhi. 2018 Feb 13;98(7):514-518. doi: 10.3760/cma.j.issn.0376-2491.2018.07.006.
To investigate the changes of CD71(+) nucleated erythrocyte apoptosis, cytochrome C (Cyt-C) and mitochondrial membrane potential (MMP) in bone marrow of chronic mountain sickness (CMS). 14 patients with CMS and 15 patients with simple old fracture were divided into CMS group and control group, respectively.Bone marrow mononuclear cells (BMMNC) were separated, marked with CD71 monoclonal antibody and stained with Annexin V-FITC/PI.Then the apoptotic index of CD71(+) nucleated erythrocytes was determined by flow cytometry.CD71(+) nucleated erythrocytes were sorted out by magnetic column separation, and Cyt-C mRNA was detected by RT-qPCR, MMP was detected by JC-1 staining flow cytometry. The apoptotic index of CD71(+) nucleated erythrocytes was (1.9±1.4)% in the CMS group, and was (3.2±1.5)% in the control group, with significant difference between the two groups (<0.05). The expression of Cyt-C mRNA was (0.72±0.14) in the CMS group, and was (1.00±0.15) in the control group, with significant difference between the two groups (<0.01). The MMP was (5.0±2.2) in the CMS group, and was (3.3±0.9) in the control group, with significant difference between the two groups (<0.05). The apoptotic index of CD71(+) nucleated erythrocytes was negatively correlated with hemoglobin in CMS group (=-0.569, =0.034). But there was no significant correlation among apoptosis index, MMP and Cyt-C mRNA. The apoptosis index of CD71(+) nucleated erythrocytes decreased in CMS patients, which was negatively correlated with the level of hemoglobin, indicating that the decline of apoptosis index of CD71(+) nucleated erythrocytes may be related to the accumulation of red blood cells in CMS.The MMP increased and Cyt-C mRNA expression decreased in CD71(+) nucleated erythrocytes of CMS patients, which suggests that the change of mitochondrial pathway of apoptosis might be involved in the down-regulation of CD71(+) nucleated erythrocytes apoptosis in CMS patients.But there was no significant correlation among CD71(+) nucleated erythrocyte apoptosis index, MMP and Cyt-C mRNA levels, which indicates that the mechanism of CD71(+) nucleated erythrocytes apoptosis is complex in CMS.
探讨慢性高原病（CMS）患者骨髓中CD71(+)有核红细胞凋亡、细胞色素C（Cyt-C）及线粒体膜电位（MMP）的变化。将14例CMS患者和15例单纯陈旧性骨折患者分别分为CMS组和对照组。分离骨髓单个核细胞（BMMNC），用CD71单克隆抗体标记，并用Annexin V-FITC/PI染色。然后通过流式细胞术检测CD71(+)有核红细胞的凋亡指数。通过磁珠分选法分选CD71(+)有核红细胞，采用RT-qPCR检测Cyt-C mRNA，采用JC-1染色流式细胞术检测MMP。CMS组CD71(+)有核红细胞凋亡指数为（1.9±1.4）%，对照组为（3.2±1.5）%，两组间差异有统计学意义（<0.05）。CMS组Cyt-C mRNA表达为（0.72±0.14），对照组为（1.00±0.15），两组间差异有统计学意义（<0.01）。CMS组MMP为（5.0±2.2），对照组为（3.3±0.9），两组间差异有统计学意义（<0.05）。CMS组CD71(+)有核红细胞凋亡指数与血红蛋白呈负相关（=-0.569，=0.034）。但凋亡指数、MMP与Cyt-C mRNA之间无显著相关性。CMS患者CD71(+)有核红细胞凋亡指数降低，且与血红蛋白水平呈负相关，提示CD71(+)有核红细胞凋亡指数下降可能与CMS患者红细胞堆积有关。CMS患者CD71(+)有核红细胞MMP升高，Cyt-C mRNA表达降低，提示凋亡线粒体途径的改变可能参与了CMS患者CD71(+)有核红细胞凋亡的下调。但CD71(+)有核红细胞凋亡指数、MMP与Cyt-C mRNA水平之间无显著相关性，表明CMS患者CD71(+)有核红细胞凋亡机制复杂。
