Cai Yu-liang, Cui Sen, Li Zhan-quan, Wang Hong-xin, Ji Lin-hua, Chai Ke-xia
Department of Hematology, Affiliated Hospital of Qinghai University, Xining, China.
Zhonghua Xue Ye Xue Za Zhi. 2011 Nov;32(11):762-5.
To observe the expressions of caspase-8 and caspase-9 mRNA, and explore the changes of apoptosis of bone marrow hematopoietic cells in patients with chronic mountain sickness (CMS).
Of 18 CMS patients and 16 controls were enrolled in this study. The apoptotic index (AI) of bone marrow mononuclear cells (BMMNC) was measured by TUNEL technique, the levels of caspase-8 and caspase-9 mRNA in BMMNC of CMS patients and controls were determined by RT-PCR. Results (1)The AI of BMMNC in patients with CMS (8.51 ± 3.35)% was lower than that in controls (16.00 ± 4.28)% (P < 0.01); (2) The values of caspase-8 and caspase-9 mRNA were (0.28 ± 0.07) and (0.23 ± 0.08) respectively, in CMS patients, which were significantly lower than those of (0.45 ± 0.09) and (0.41 ± 0.09) respectively, in the controls (both P < 0.01); (3) Hemoglobin (Hb) value was negatively correlated with levels of caspase-8 and caspase-9 mRNA (r values were -0.52 and -0.61 respectively, both P < 0.05) in CMS patients. There was a negative correlation between AI and Hb (r value was -0.89, P < 0.01) in CMS patients. However, the significant relationship was not found between AI and level of caspase-8 or caspase-9 mRNA (P > 0.05).
The results showed a decrease apoptosis of BMMNCs and reduced levels of caspase-8 and caspase-9 mRNA in CMS patients, the latter might be involved in the change of BMMNCs apoptosis.
观察慢性高原病（CMS）患者骨髓造血细胞中半胱天冬酶 -8（caspase-8）和半胱天冬酶 -9（caspase-9）mRNA 的表达，探讨其细胞凋亡的变化。
选取 18 例 CMS 患者和 16 例对照者。采用 TUNEL 技术检测骨髓单个核细胞（BMMNC）的凋亡指数（AI），应用 RT-PCR 法检测 CMS 患者及对照者 BMMNC 中 caspase-8 和 caspase-9 mRNA 的水平。结果 （1）CMS 患者 BMMNC 的 AI 为（8.51±3.35）%，低于对照组的（16.00±4.28）%（P<0.01）；（2）CMS 患者 caspase-8 和 caspase-9 mRNA 的值分别为（0.28±0.07）和（0.23±0.08），显著低于对照组的（0.45±0.09）和（0.41±0.09）（均 P<0.01）；（3）CMS 患者血红蛋白（Hb）值与 caspase-8 和 caspase-9 mRNA 水平呈负相关（r 值分别为 -0.52 和 -0.61，均 P<0.05）。CMS 患者 AI 与 Hb 呈负相关（r 值为 -0.89，P<0.01）。然而，未发现 AI 与 caspase-8 或 caspase-9 mRNA 水平之间存在显著相关性（P>0.05）。
结果显示 CMS 患者 BMMNC 凋亡减少，caspase-8 和 caspase-9 mRNA 水平降低，后者可能参与了 BMMNC 凋亡的变化。
