Yang Song, Yan Tao, Zhao Liulan, Wu Hao, Du Zongjun, Yan Taiming, Xiao Qing
College of Animal Science and Technology, Sichuan Agricultural University, Wen Jiang, Sichuan, 611130, China.
College of Animal Science and Technology, Sichuan Agricultural University, Wen Jiang, Sichuan, 611130, China.
J Therm Biol. 2018 Feb;72:155-160. doi: 10.1016/j.jtherbio.2018.02.005. Epub 2018 Feb 8.
This study evaluated the effects of temperature on the activities of antioxidant enzymes and hormone levels in Schizothorax prenanti. Schizothorax prenanti were acutely suffered from increasing temperature at a rate of 1 °C h. The temperature started from 11 °C and the critical thermal maximum (CTMax) was measured to evaluated thermal tolerance of Schizothorax prenanti. Antioxidant parameters including superoxide dismutase (SOD), catalase (CAT), Na/K-ATPase and malondialdehyde (MDA) in gills, liver and muscle were measured at five temperature groups (11 °C, 16 °C, 21 °C, 26 °C and 31 °C). The plasma hormone including acetylcholine (ACh) and cortisol were also measured at five temperature groups. Our results showed that the CTMax of Schizothorax prenanti under acute heat stress was 31 °C.The activities of SOD and CAT in liver and muscle at 11 °C, 16 °C, 26 °C and 31 °C groups were significantly higher than 21 °C group, as well as Na/K-ATPase in gills and MDA concentrations in gills and liver. However, plasma ACh and cortisol levels were significantly increased with increasing temperature. The results indicate that oxidative stress parameters could respons to increase of temperatur, altogether reflect that Schizothorax prenanti has higher susceptibility of temperature. Thus, the effect of long-term high temperature on Schizothorax prenanti should be studied further.
本研究评估了温度对齐口裂腹鱼抗氧化酶活性和激素水平的影响。齐口裂腹鱼以1℃/h的速率急性遭受温度升高。温度从11℃开始，测量临界热最大值（CTMax）以评估齐口裂腹鱼的热耐受性。在五个温度组（11℃、16℃、21℃、26℃和31℃）下测量鳃、肝脏和肌肉中的抗氧化参数，包括超氧化物歧化酶（SOD）、过氧化氢酶（CAT）、钠钾ATP酶和丙二醛（MDA）。在五个温度组下还测量了血浆激素，包括乙酰胆碱（ACh）和皮质醇。我们的结果表明，急性热应激下齐口裂腹鱼的CTMax为31℃。11℃、16℃、26℃和31℃组肝脏和肌肉中的SOD和CAT活性显著高于21℃组，鳃中的钠钾ATP酶以及鳃和肝脏中的MDA浓度也是如此。然而，血浆ACh和皮质醇水平随温度升高而显著增加。结果表明，氧化应激参数可对温度升高作出反应，总体反映出齐口裂腹鱼对温度具有较高的敏感性。因此，长期高温对齐口裂腹鱼的影响有待进一步研究。
