一种用于筛选产16S rRNA甲基化酶的泛氨基糖苷耐药革兰氏阴性菌的培养基。
A culture medium for screening 16S rRNA methylase-producing pan-aminoglycoside resistant Gram-negative bacteria.
作者信息
Nordmann Patrice, Mazé Alain, Culebras Esther, Dobias Jan, Jayol Aurélie, Poirel Laurent
机构信息
Medical and Molecular Microbiology Unit, Department of Medicine, Faculty of Science, University of Fribourg, Fribourg, Switzerland; INSERM European Unit (LEA, IAME, France), University of Fribourg, Fribourg, Switzerland; Swiss National Reference Center for Emerging Antibiotic Resistance (NARA), Fribourg, Switzerland; Institute for Microbiology, University of Lausanne and University Hospital Centre, Lausanne, Switzerland.
Medical and Molecular Microbiology Unit, Department of Medicine, Faculty of Science, University of Fribourg, Fribourg, Switzerland; INSERM European Unit (LEA, IAME, France), University of Fribourg, Fribourg, Switzerland.
出版信息
Diagn Microbiol Infect Dis. 2018 Jun;91(2):118-122. doi: 10.1016/j.diagmicrobio.2018.01.026. Epub 2018 Feb 3.
The amikacin plus gentamicin-containing SuperAminoglycoside medium was developed for screening multiple-aminoglycoside resistance in Gram-negative bacteria (Enterobacteriaceae, Pseudomonas aeruginosa, and Acinetobacter baumannii). It was evaluated using aminoglycoside-susceptible (n=12) and aminoglycoside-resistant (n=59) Gram-negative isolates, including 16S rRNA methylase producers (n=20). Its sensitivity and specificity of detection were, respectively, of 95% and 96% for detecting multiple aminoglycoside-resistant methylase producers.
含阿米卡星和庆大霉素的超级氨基糖苷培养基是为筛选革兰氏阴性菌（肠杆菌科、铜绿假单胞菌和鲍曼不动杆菌）对多种氨基糖苷的耐药性而研制的。使用对氨基糖苷敏感的革兰氏阴性菌分离株（n = 12）和对氨基糖苷耐药的革兰氏阴性菌分离株（n = 59）对其进行了评估，其中包括16S rRNA甲基化酶产生菌（n = 20）。在检测对多种氨基糖苷耐药的甲基化酶产生菌方面，其检测灵敏度和特异性分别为95%和96%。
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