脑脊液的定量蛋白质组学揭示了鼻腔型结外 NK/T 细胞淋巴瘤伴筛窦转移的潜在预测性生物标志物。
Quantitative proteomics of CSF reveals potential predicted biomarkers for extranodal NK-/T-cell lymphoma of nasal-type with ethmoidal sinus metastasis.
机构信息
Cancer Center, State Key Laboratory of Biotherapy, West China Hospital, Sichuan University, Chengdu 610041, China.
West China School of Medicine, West China Hospital, Sichuan University, Chengdu 610041, China.
出版信息
Life Sci. 2018 Apr 1;198:94-98. doi: 10.1016/j.lfs.2018.02.035. Epub 2018 Feb 26.
AIM
Extranodal natural killer cell/T-cell lymphoma of nasal-type (NKTCL) is an aggressive human lymphoma, but its predicted biomarkers after chemotherapy are less known. The aim of this study is to find some potential predicted biomarkers in cerebrospinal fluid (CSF) of NKTCL patients with ethmoidal sinus metastasis (NESM).
MATERIALS AND METHODS
The CSF samples were obtained from NKTCL patients with NESM before and after chemotherapy from Cancer Center of West China Hospital. Comparative proteomic profiling using label-free method was performed to characterize the fold change of proteins in NESM patients.
KEY FINDING
In this study, 102 proteins with <1% false discovery rate in CSF of NKTCL with NESM patients were quantified. Furthermore, significantly reduced IGFBP2, SERP1NC1, AMBP and GPX3, as well as dramatically increased CPE levels were observed in the CSF of NKTCL patients after cytarabine chemotherapy.
SIGNIFICANCE
IGFBP2, SERP1NC1, AMBP, GPX3 and CPE together or alone have a potential to be predicted indicators of NKTCL with NESM in response to chemotherapy.
目的
鼻型结外自然杀伤细胞/T 细胞淋巴瘤（NKTCL）是一种侵袭性人类淋巴瘤，但对其化疗后预测生物标志物知之甚少。本研究旨在寻找 NKTCL 伴筛窦转移（NESM）患者脑脊液（CSF）中一些潜在的预测生物标志物。
材料和方法
本研究采用无标记法比较蛋白质组学分析，对来自华西医院肿瘤中心的 NESM 化疗前后的 NKTCL 患者的 CSF 样本进行了分析。
主要发现
在这项研究中，定量了 NESM 患者 CSF 中 102 种假发现率<1%的蛋白质。此外，在阿糖胞苷化疗后，NKTCL 患者的 CSF 中 IGFBP2、SERP1NC1、AMBP 和 GPX3 显著降低，CPE 水平显著升高。
意义
IGFBP2、SERP1NC1、AMBP、GPX3 和 CPE 单独或联合具有作为预测 NESM 对化疗反应的 NKTCL 潜在指标的潜力。
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