Li Qingfang, Zeng Hao, Zhao Yunuo, Gong Yanqiu, Ma Xuelei
State Key Laboratory of Biotherapy, Department of Biotherapy, Cancer Center, West China Hospital, Sichuan University, Chengdu, China.
Front Oncol. 2020 Jan 10;9:1489. doi: 10.3389/fonc.2019.01489. eCollection 2019.
Extranodal natural killer /T-cell lymphoma (ENKTL) is an aggressive and unusual subtype of non-Hodgkin lymphoma (NHL) that it is related with the Epstein-Barr virus (EBV). CSF is considered as an ideal source of high-concenrtation disease-related proteins. We aimed at identifying the proteomic markers changes of CSF in ENKTL patients and used such changes to diagnose ENKTL. In this study, CSF samples were acquired from hospitalization patients from the Cancer Center of West China Hospital, Chengdu, China. Comparative proteomic profiling are commonly used to do label-free liquid chromatography-tandem mass spectrometry (LC-MS/MS). And in this study the same method was used to characterize the variety of proteins in ENKTL patients and none-ENKTL people. In the aggregate, 421 non-excrescent and functional proteins were identified among the samples. Of these proteins, 45 proteins quantified match the involved criteria. HRG, TIMP-1, SERPINA3, FGA, FGG, TF, FGB, APP, and AGT were significantly up-regulated. We discovered that some proteins were significantly up-regulated. Also, these proteins themselves or with others proteins may be potential markers to diagnose ENKTL. The changes of proteomics may be a potential method to precisely identify the pathogenesis of the ENKTL.
结外自然杀伤/T细胞淋巴瘤（ENKTL）是一种侵袭性且罕见的非霍奇金淋巴瘤（NHL）亚型，与爱泼斯坦-巴尔病毒（EBV）相关。脑脊液被认为是高浓度疾病相关蛋白的理想来源。我们旨在鉴定ENKTL患者脑脊液中的蛋白质组学标志物变化，并利用这些变化来诊断ENKTL。在本研究中，脑脊液样本取自中国成都华西医院癌症中心的住院患者。比较蛋白质组分析通常用于无标记液相色谱-串联质谱（LC-MS/MS）。在本研究中，采用相同方法对ENKTL患者和非ENKTL患者的多种蛋白质进行表征。总共在样本中鉴定出421种无异常且有功能的蛋白质。在这些蛋白质中，45种定量蛋白质符合相关标准。HRG、TIMP-1、SERPINA3、FGA、FGG、TF、FGB、APP和AGT显著上调。我们发现一些蛋白质显著上调。此外，这些蛋白质本身或与其他蛋白质一起可能是诊断ENKTL的潜在标志物。蛋白质组学的变化可能是精确识别ENKTL发病机制的一种潜在方法。
