University of Münster, Institute of Pharmaceutical Biology and Phytochemistry, Corrensstrasse 48, D-48149 Münster, Germany.
University of Münster, Institute of Biology and Plant Biotechnology, Schlossgarten 3, D-48149 Münster, Germany.
Fitoterapia. 2018 Jun;127:237-244. doi: 10.1016/j.fitote.2018.02.029. Epub 2018 Feb 26.
Fruits from Apium graveolens (Celery) are used traditionally in Persian and European medicine for the treatment of uncomplicated urinary tract infections. No data are available on A. graveolens extract on the interplay between uropathogenic E. coli and the eukaryotic host cells and on quorum sensing of the bacteria. The present study aimed to characterize an antiadhesive and anti quorum sensing effect of a characterized A. graveolens extract by specific in vitro assays and to correlate these effects with in vivo data obtained by an animal infection model. Hydroalcoholic extract CSE (EtOH-water, 1:1) from A. graveolens fruits was characterized by UHPLC/+ESI-QTOF-MS and investigated on antiproliferative activity against UPEC (strain NU14) and human T24 bladder cells. Antiadhesive properties of CSE were investigated within two different in vitro adhesion assays. For in vivo studies BALB/c mice were used in an UPEC infection model. The effect of CSE on bacterial load in bladder tissue was monitored within a 4- and 7 days pretreatment (200, 500 mg/kg) of the animals. CSE was dominated by the presence of luteolin-glycosides and related flavons besides furocoumarins. CSE had no cytotoxic effects against UPEC and bladder cells. CSE exerts a dose dependent antiadhesive activity against UPEC strains NU14 and UTI89. CSE inhibited in a concentration-dependent manner bacterial quorum sensing. 4- and 7-day pretreatment of animals with CSE transurethrally infected with UPEC NU14, significantly reduced the bacterial load in bladder tissue. CSE is assessed as an antiadhesive extract for which the traditional use in phytotherapy for UTI is justified.
芹菜果实被广泛应用于传统的波斯和欧洲医学，用于治疗简单的尿路感染。目前还没有关于芹菜提取物在尿路致病性大肠杆菌与真核宿主细胞相互作用以及细菌群体感应方面的研究数据。本研究旨在通过特定的体外试验，对已鉴定的芹菜提取物的抗黏附作用和抗群体感应作用进行表征，并将这些作用与动物感染模型获得的体内数据相关联。芹菜果实的水醇提取物 CSE（乙醇-水，1:1）通过 UHPLC/+ESI-QTOF-MS 进行了表征，并针对其对 UPEC（NU14 株）和人 T24 膀胱细胞的抗增殖活性进行了研究。在两种不同的体外黏附试验中研究了 CSE 的抗黏附特性。对于体内研究，使用 BALB/c 小鼠进行了 UPEC 感染模型的研究。在动物预处理（200、500mg/kg）4 天和 7 天内，监测 CSE 对膀胱组织中细菌负荷的影响。CSE 以叶黄素糖苷和相关黄酮类化合物以及呋喃香豆素为主要成分。CSE 对 UPEC 和膀胱细胞没有细胞毒性作用。CSE 对 UPEC 菌株 NU14 和 UTI89 表现出剂量依赖性的抗黏附活性。CSE 以浓度依赖性方式抑制细菌群体感应。用 CSE 经尿道预处理 4 天和 7 天的经尿道感染 UPEC NU14 的动物，膀胱组织中的细菌负荷显著降低。CSE 被评估为一种具有抗黏附作用的提取物，其在植物疗法中用于治疗尿路感染是合理的。
