Popper Bastian, Demleitner Antonia, Bolivar Valerie J, Kusek Gretchen, Snyder-Keller Abigail, Schieweck Rico, Temple Sally, Kiebler Michael A
Biomedical Center (BMC), Department for Cell Biology & Anatomy, Medical Faculty, Ludwig-Maximilians-University, Munich, Germany; Biomedical Center (BMC), Core Facility Animal Models, Ludwig-Maximilians-University, Munich, Germany.
Biomedical Center (BMC), Department for Cell Biology & Anatomy, Medical Faculty, Ludwig-Maximilians-University, Munich, Germany.
Neurobiol Learn Mem. 2018 Apr;150:107-115. doi: 10.1016/j.nlm.2018.02.027. Epub 2018 Feb 26.
Staufen2 (Stau2) is a double-stranded RNA-binding protein (RBP) involved in posttranscriptional gene expression control in neurons. In flies, staufen contributes to learning and long-term memory formation. To study the impact of mammalian Stau2 on behavior, we generated a novel gene-trap mouse model that yields significant constitutive downregulation of Stau2 (Stau2). In order to investigate the effect of Stau2 downregulation on hippocampus-dependent behavior, we performed a battery of behavioral assays, i.e. open field, novel object recognition/location (NOR/L) and Barnes maze. Stau2 mice displayed reduced locomotor activity in the open field and altered novelty preference in the NOR and NOL paradigms. Adult Stau2 male mice failed to discriminate between familiar and newly introduced objects but showed enhanced spatial novelty detection. Additionally, we observed deficits in discriminating different spatial contexts in a Barnes maze assay. Together, our data suggest that Stau2 contributes to novelty preference and explorative behavior that is a driver for proper spatial learning in mice.
Staufen2（Stau2）是一种双链RNA结合蛋白（RBP），参与神经元转录后基因表达的调控。在果蝇中，Staufen有助于学习和长期记忆的形成。为了研究哺乳动物Stau2对行为的影响，我们构建了一种新型基因陷阱小鼠模型，该模型可导致Stau2（Stau2）显著的组成性下调。为了研究Stau2下调对海马体依赖性行为的影响，我们进行了一系列行为学实验，即旷场实验、新物体识别/定位（NOR/L）实验和巴恩斯迷宫实验。Stau2小鼠在旷场实验中表现出运动活性降低，在NOR和NOL实验范式中改变了新奇偏好。成年Stau2雄性小鼠无法区分熟悉的物体和新引入的物体，但表现出增强的空间新奇性检测能力。此外，我们在巴恩斯迷宫实验中观察到辨别不同空间环境的缺陷。总之，我们的数据表明，Stau2有助于新奇偏好和探索行为，而这是小鼠进行适当空间学习的驱动力。
