Biomedical Center (BMC), Department for Cell Biology & Anatomy, Medical Faculty, Ludwig-Maximilians- University, 82152 Martinsried, Germany.
Institute of Clinical Neuroimmunology, University Hospital and Biomedical Center, Ludwig-Maximilians-University, 81377 Munich, Germany.
Int J Mol Sci. 2019 Apr 11;20(7):1797. doi: 10.3390/ijms20071797.
Staufen2 (Stau2) is an RNA-binding protein that is involved in dendritic spine morphogenesis and function. Several studies have recently investigated the role of Stau2 in the regulation of its neuronal target mRNAs, with particular focus on the hippocampus. Here, we provide evidence for Stau2 expression and function in cerebellar Purkinje cells. We show that Stau2 downregulation (Stau2) led to an increase of glutamate receptor ionotropic delta subunit 2 (GluD2) in Purkinje cells when animals performed physical activity by voluntary wheel running compared with the age-matched wildtype (WT) mice (C57Bl/6J). Furthermore, Stau2 mice showed lower performance in motor coordination assays but enhanced motor learning abilities than did WT mice, concomitantly with an increase in dendritic GluD2 expression. Together, our results suggest the novel role of Stau2 in Purkinje cell synaptogenesis in the mouse cerebellum.
Staufen2（Stau2）是一种 RNA 结合蛋白，参与树突棘形态发生和功能。最近有几项研究探讨了 Stau2 在调节其神经元靶 mRNA 中的作用，特别是在海马体中。在这里，我们提供了 Stau2 在小脑浦肯野细胞中表达和功能的证据。我们发现，与年龄匹配的野生型（WT）小鼠（C57Bl/6J）相比，当动物通过自愿轮跑进行体育活动时，Stau2 下调（Stau2）导致浦肯野细胞中谷氨酸受体离子型 δ 亚基 2（GluD2）增加。此外，Stau2 小鼠在运动协调测试中的表现较差，但比 WT 小鼠具有更强的运动学习能力，同时伴随着树突 GluD2 表达的增加。总之，我们的结果表明 Stau2 在小鼠小脑浦肯野细胞突触发生中的新作用。
