Käbisch S, Zitnik B, Krumholz W, Lohmeyer J, Pralle H, Biscoping J, Hempelmann G
Anasth Intensivther Notfallmed. 1986 Dec;21(6):327-32.
44 patients undergoing comparable gynaecological operations received either halothane- modified neuroleptanaesthesia or a combination of epidural and NLA. Monoclonal antibodies were used to determine 8 different lymphocyte subpopulations in blood samples drawn before and after anaesthesia and on the first postoperative day. Cell populations were counted by fluorescent microscopy. Halothane anaesthesia produced both a depression of T-cells (72.67% preoperatively to 52.79% postoperatively) and of the helper/suppressor ratio (1.46 to 1.10); meanwhile activated T-cells increased from 2.33% to 6.83%. After neuroleptanaesthesia as well as after halothane the HLA-DR positive cells decreased (21.05% to 19.29%; 25.22% to 20.29%). The B-cell fraction was elevated from 2.33% to 6.83% following combined anaesthesia. The potential of the anaesthetics to produce subsequent alterations in host defense is discussed.
44名接受类似妇科手术的患者接受了氟烷改良神经安定麻醉或硬膜外麻醉与神经安定麻醉的联合麻醉。使用单克隆抗体测定麻醉前、麻醉后及术后第一天采集的血样中的8种不同淋巴细胞亚群。通过荧光显微镜对细胞群体进行计数。氟烷麻醉导致T细胞减少（术前72.67%降至术后52.79%）以及辅助/抑制细胞比例降低（从1.46降至1.10）；同时，活化T细胞从2.33%增加至6.83%。神经安定麻醉后以及氟烷麻醉后，HLA-DR阳性细胞均减少（从21.05%降至19.29%；从25.22%降至20.29%）。联合麻醉后B细胞比例从2.33%升高至6.83%。文中讨论了麻醉剂对宿主防御产生后续改变的可能性。
