Identification and isolation of vitamin K-dependent proteins by HPLC.

Six of the seven known vitamin K-dependent proteins found in plasma were chromatographed on a large-pore propylsilane column using aqueous trifluoroacetic acid/acetonitrile gradients. Prothrombin and Factor VII coeluted, the others were readily resolved. The technique has been used to monitor the purification of protein C and protein S using immobilized anti-protein S. Preliminary evidence is presented which is suggestive of the existence of additional vitamin K-dependent proteins in plasma.