Department of Traditional Chinese Medicine, The First Affiliated Hospital of Anhui Medical University, Hefei, Anhui 230022, People's Republic of China; Anhui University of Chinese Medicine, Hefei, People's Republic of China.
Scientific Research Experiment Center, Anhui University of Chinese Medicine, Hefei, People's Republic of China.
Biomed Pharmacother. 2018 May;101:311-321. doi: 10.1016/j.biopha.2018.02.028. Epub 2018 Mar 22.
Although the effect of Huaier has been widely studied, its role and its molecular mechanism in lung cancer are not clear. In this study, we explored the inhibitory effect of Huaier on lung cancer cells and its molecular mechanism. The cell viability, migration and invasion were analyzed by CCK-8 and BrdU cell proliferation assay kits, Transwell and colony forming assay. The cell cycle and apoptosis were analyzed by flow cytometry. The experimental results showed that the viability, migration and invasion of A549 and NCI-H1650 cells were inhibited by Huaier in a dose and time-dependent manner. Huaier induced cell apoptosis and the cells were blocked in the S phase to inhibit cell proliferation. Western blotting results showed that Huaier inhibited the expression of MTDH and increased the proportion of Bax/Bcl-2, it could also promote the expression of Cleaved Caspase-3 and increase the activity of Caspase-3, promote cell apoptosis and inhibit cell proliferation. Huaier inhibited the metastasis and invasion of lung cancer cells by inhibiting the expression levels of EMT related proteins, it also inhibited the expression of JAK2/STAT3 and MAPK signaling pathways. Therefore, our results showed that Huaier may inhibit the proliferation and metastasis of lung cancer cells through multiple targets, it had the potential for treatment of lung cancer.
尽管已有大量研究探讨了槐耳的作用，但槐耳在肺癌中的作用及其分子机制尚不清楚。本研究旨在探讨槐耳对肺癌细胞的抑制作用及其分子机制。通过 CCK-8 和 BrdU 细胞增殖试剂盒、Transwell 和集落形成实验分析细胞活力、迁移和侵袭。通过流式细胞术分析细胞周期和细胞凋亡。实验结果表明，槐耳呈剂量和时间依赖性地抑制 A549 和 NCI-H1650 细胞的活力、迁移和侵袭。槐耳诱导细胞凋亡，并将细胞阻滞在 S 期以抑制细胞增殖。Western blot 结果表明，槐耳抑制 MTDH 的表达，增加 Bax/Bcl-2 的比例，还能促进 Cleaved Caspase-3 的表达，增加 Caspase-3 的活性，促进细胞凋亡，抑制细胞增殖。槐耳通过抑制 EMT 相关蛋白的表达水平抑制肺癌细胞的转移和侵袭，还抑制 JAK2/STAT3 和 MAPK 信号通路的表达。因此，我们的研究结果表明，槐耳可能通过多个靶点抑制肺癌细胞的增殖和转移，具有治疗肺癌的潜力。
