University of Limerick, Faculty of Science and Engineering, Department of Biological Sciences, Ireland; University of Limerick, Faculty of Science and Engineering, Department of Mathematics and Statistics, Ireland.
University of Limerick, Faculty of Science and Engineering, Department of Mathematics and Statistics, Ireland; University of Oxford, OCIAM, Mathematical Institute, United Kingdom.
Sci Total Environ. 2018 Jul 15;630:849-858. doi: 10.1016/j.scitotenv.2018.02.263. Epub 2018 Feb 27.
This study evaluated the effect of one-time phosphate fertilization on the soil microbiota, its cycling of phosphorus (P) and grass growth. Soil columns were established in a greenhouse using a P-limited Irish soil (index 1), planted with Lolium perenne and fertilized with 0 (control), 5 (quarter), 10 (half) and 20 (full)kgPha as inorganic phosphate. Only traces of phosphate in soil solution were detected over the 14week experiment, even after phosphate fertilization. Grass dry matter yield between treatments was not significantly different. Full phosphate fertilization significantly reduced the arbuscular mycorrhization (AM) rate, bacterial- and fungal-feeding nematode population, bacterial phoD gene abundance, but increased alkaline and acid phosphatase activities at the time of harvest. Full and half P treatments significantly shifted the bacterial, fungal and AM community structures compared to the control. Furthermore, the control had a significantly higher relative abundance of bacterial genera including Bacillus, Bradyrhizobium, Paenibacillus, Nocardioides and Balneimonas, that have been associated with P mobilization in the past, when compared to the full phosphate treatment. These results suggest that a positive effect of a single phosphate application on plant growth in a soil can be cancelled out by its negative effect on the soil microbiota and their ecosystem services.
本研究评估了一次性施加磷肥对土壤微生物群落、磷循环和草生长的影响。使用一种磷限制的爱尔兰土壤（指数 1）在温室中建立了土壤柱，种植黑麦草，并以 0（对照）、5（四分之一）、10（一半）和 20（全量）kgPha 的无机磷酸盐进行施肥。即使在施加磷肥后，在 14 周的实验中，土壤溶液中也只检测到痕量的磷酸盐。处理之间的草干物质产量没有显著差异。全量磷肥施肥显著降低了丛枝菌根（AM）率、细菌和真菌取食线虫种群、细菌 phoD 基因丰度，但在收获时增加了碱性和酸性磷酸酶活性。与对照相比，全量和半量 P 处理显著改变了细菌、真菌和 AM 群落结构。此外，与全磷处理相比，对照具有显著更高的细菌属的相对丰度，包括过去与磷动员有关的芽孢杆菌、慢生根瘤菌、类芽孢杆菌、诺卡氏菌和巴尔内姆氏菌。这些结果表明，土壤中单次施加磷肥对植物生长的积极影响可能被其对土壤微生物群落及其生态系统服务的负面影响所抵消。
