Department of Instrumentation & Analytical Chemistry, CAS Key Laboratory of Separation Sciences for Analytical Chemistry, Dalian Institute of Chemical Physics, Chinese Academy of Sciences, 457 Zhongshan Road, Dalian 116023, China.
Department of Instrumentation & Analytical Chemistry, CAS Key Laboratory of Separation Sciences for Analytical Chemistry, Dalian Institute of Chemical Physics, Chinese Academy of Sciences, 457 Zhongshan Road, Dalian 116023, China; University of Chinese Academy of Sciences, Beijing 100049, PR China.
Talanta. 2018 May 15;182:279-284. doi: 10.1016/j.talanta.2018.01.076. Epub 2018 Feb 20.
A compact and low-cost laser induced fluorescence (LIF) detector based on confocal structure for capillary flow systems was developed and applied for analysis of Her2 protein on single Hela cells. A low-power and low-cost 450 nm laser diode (LD) instead of a high quality laser was used as excitation light source. A compact optical design together with shortened optical path length improved the optical efficiency and detection sensitivity. A superior silicon based photodetector assembly was used for fluorescence detection instead of a photomultiplier (PMT). The limit of detection (LOD) for fluorescein sodium was 3 × 10 M or 165 fluorescein molecules in detection volume measured on a homemade capillary electroosmotic driven (EOD)-LIF system, which was similar to commercial LIFs. Compared to commercial LIFs, the whole volume of our LIF was reduced to 1/2-1/3, and the cost was less than 1/3 of them.
一种基于共焦结构的紧凑型低成本激光诱导荧光（LIF）检测器，用于毛细管流动系统，用于分析单个 Hela 细胞上的 Her2 蛋白。使用低功率、低成本的 450nm 激光二极管（LD）代替高质量激光作为激发光源。紧凑的光学设计和缩短的光路长度提高了光学效率和检测灵敏度。使用基于硅的高性能光电探测器组件代替光电倍增管（PMT）进行荧光检测。在自制的毛细管电动驱动（EOD）-LIF 系统上，对荧光素钠的检测限（LOD）为 3×10^-7M 或 165 个荧光素分子，与商用 LIF 相当。与商用 LIF 相比，我们的整个 LIF 体积缩小到 1/2-1/3，成本不到它们的 1/3。
