使用基质辅助激光解吸电离生物分型抗生素敏感性试验快速检测法（MBT-ASTRA）对脆弱拟杆菌进行药敏试验。
Antimicrobial susceptibility testing of Bacteroides fragilis using the MALDI Biotyper antibiotic susceptibility test rapid assay (MBT-ASTRA).
作者信息
Justesen Ulrik Stenz, Acar Ziyap, Sydenham Thomas Vognbjerg, Johansson Åsa
机构信息
Department of Clinical Microbiology, Odense University Hospital, Odense, Denmark.
Department of Clinical Microbiology, Odense University Hospital, Odense, Denmark.
出版信息
Anaerobe. 2018 Dec;54:236-239. doi: 10.1016/j.anaerobe.2018.02.007. Epub 2018 Mar 2.
This study evaluated the MBT-ASTRA for antimicrobial susceptibility testing of Bacteroides fragilis with different classes of antibiotics. MALDI-TOF MS peak AUCs from suspensions with B. fragilis with and without an antibiotic were used to calculate the relative growth (AUC "with antibiotic" divided by "without antibiotic"). Antimicrobial susceptibility testing of B. fragilis ATCC 25285 (susceptible) and B. fragilis O18 (resistant) was demonstrated with a clear difference of the relative growth between susceptible and resistant. The MBT-ASTRA needs further development and assessment but could be a relatively easy and inexpensive method for rapid antimicrobial susceptibility testing in specific cases of infection with B. fragilis.
本研究评估了MBT-ASTRA用于脆弱拟杆菌对不同种类抗生素的药敏试验。来自含有和不含抗生素的脆弱拟杆菌悬液的基质辅助激光解吸电离飞行时间质谱（MALDI-TOF MS）峰面积曲线下面积（AUC）用于计算相对生长率（“有抗生素”的AUC除以“无抗生素”的AUC）。对脆弱拟杆菌ATCC 25285（敏感株）和脆弱拟杆菌O18（耐药株）进行的药敏试验表明，敏感株和耐药株之间的相对生长率有明显差异。MBT-ASTRA需要进一步开发和评估，但在脆弱拟杆菌特定感染病例中，可能是一种相对简便且廉价的快速药敏试验方法。
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