Department of Cardiovascular Medicine, The Affiliated Hospital of Qingdao University, Qingdao, Shandong Province, 266000 China.
Department of Cardiovascular Medicine, The Affiliated Hospital of Qingdao University, Qingdao, Shandong Province, 266000 China.
Hellenic J Cardiol. 2018 Sep-Oct;59(5):274-278. doi: 10.1016/j.hjc.2018.02.009. Epub 2018 Mar 6.
This study sought to investigate the effects of Guanfu total base on Bcl-2 and Bax expression and the correlation of Bcl-2 and Bax expression with atrial fibrillation.
Twenty-four male Sprague-Dawley rats were randomly divided into control, model, and treatment groups (n = 8 each). A combined intravenous injection of CaCl (10 mg/mL) and acetylcholine (Ach; 66 μg/mL) was administered to the model and treatment groups for 7 consecutive days to induce atrial fibrillation. After 3 days, the treatment group was administered orally with Aconitum coreanum. Controls received saline for 7 consecutive days. Atrial fibrillation duration was monitored by using an electrocardiogram. Immunohistochemical analysis was used to measure the expression and distribution of Bcl-2 and Bax in the atrial myocardial tissues, RT-PCR was used to measure the Bcl-2 and Bax mRNA expression, and western blot was used to measure Bcl-2 and Bax expression in the atrial myocardial tissue.
The model group showed prolonged atrial fibrillation, but this was absent in the control and treatment groups, indicating that treatment with A. coreanum effectively reduced atrial fibrillation duration. Immunohistochemical analysis showed that Bcl-2 expression in the atrial muscle tissue was significantly lower, but Bax expression was significantly higher in the model group compared to that in the control group. After treatment, Bcl-2 expression increased and Bax expression decreased (P < 0.01) in the treatment group compared to those in the model group. RT-PCR and western blot presented the same trends.
Bcl-2 and Bax expression was correlated with atrial fibrillation. Guanfu total base was effective in treating atrial fibrillation, upregulating Bcl-2 expression, and downregulating Bax expression.
本研究旨在探讨关附总碱对 Bcl-2 和 Bax 表达的影响，以及 Bcl-2 和 Bax 表达与心房颤动的相关性。
24 只雄性 Sprague-Dawley 大鼠随机分为对照组、模型组和治疗组（每组 8 只）。模型组和治疗组连续 7 天静脉注射氯化钙（10mg/ml）和乙酰胆碱（Ach；66μg/ml），诱发心房颤动。第 3 天，治疗组给予关附总碱灌胃，对照组连续 7 天给予生理盐水。使用心电图监测心房颤动持续时间。免疫组织化学分析测量心房心肌组织中 Bcl-2 和 Bax 的表达和分布，RT-PCR 测量 Bcl-2 和 Bax mRNA 表达，Western blot 测量心房心肌组织中 Bcl-2 和 Bax 的表达。
模型组心房颤动时间延长，但对照组和治疗组无此现象，表明用关附总碱治疗可有效减少心房颤动时间。免疫组织化学分析显示，模型组心房肌组织中 Bcl-2 表达明显降低，而 Bax 表达明显升高。治疗后，治疗组 Bcl-2 表达增加，Bax 表达降低（P<0.01），与模型组比较。RT-PCR 和 Western blot 呈现相同的趋势。
Bcl-2 和 Bax 的表达与心房颤动有关。关附总碱治疗心房颤动有效，上调 Bcl-2 表达，下调 Bax 表达。
