Niu Qiao, Wang Lin-ping, Chen Yi-lan, Zhang Hong-mei
Department of Occupational Health, Shanxi Medical University, Taiyuan 030001,China.
Wei Sheng Yan Jiu. 2005 Nov;34(6):671-3.
To explore the effects of aluminum chloride (AlCl3) on the apoptosis of rat hippocampus cells and the relationship between the apoptosis and the expression of the bcl-2 as well as bax mRNA.
40 SD rats, adult and healthy, were divided into 4 groups randomly: normal saline, Al3+ 2.5 mg/kg, 5 mg/kg, 10 mg/kg. They were exposed to AlCl, by intraperitioneal injection at the dose of 0.2 ml/d for 60 days. After exposure, the apoptosis of hippocampus cells were measured by method of TUNEL. The expression of bcl-2 mRNA and bax mRNA in rats' hippocampus were measured by RT-PCR. The results were analyzed by the gelatum analyzing system.
The rates of apoptosis in hippocampus cells in Al3+ 5 mg/kg group and Al3+ 10 mg/kg group were significantly higher than that of controls (P < 0.05). But Al3+ 2.5 mg/kg group was not significantly higher than that of control (P > 0.05), and was significantly decreased in hippocampus cells in Al3+ 5 mg/kg and 10 mg/kg group compared with the control group (P < 0.05). The expression of bcl-2 mRNA was significantly decreased in every aluminum treatment group compared with the control group (P < 0.05). The expression of bax mRNA was significantly increased in every aluminum treatment group compared with the control group (P < 0.05). There was negative correlation between AI and the expression of Bcl-2 (r = -0.909, P < 0.01). There was positive correlation between Al and the expression of Bax (r = 0.871, P < 0.01).
Aluminum can induce apoptosis in rat hippocampus cells through the downwards regulation of bcl-2 mRNA, the upwards regulation of bax mRNA expression.
探讨氯化铝（AlCl₃）对大鼠海马细胞凋亡的影响以及凋亡与bcl-2和bax mRNA表达之间的关系。
将40只成年健康SD大鼠随机分为4组：生理盐水组、2.5mg/kg Al³⁺组、5mg/kg Al³⁺组、10mg/kg Al³⁺组。以0.2ml/d的剂量腹腔注射AlCl₃，持续60天。染毒结束后，采用TUNEL法检测海马细胞凋亡情况。用RT-PCR法检测大鼠海马中bcl-2 mRNA和bax mRNA的表达。结果通过凝胶分析系统进行分析。
5mg/kg Al³⁺组和10mg/kg Al³⁺组海马细胞凋亡率显著高于对照组（P<0.05）。但2.5mg/kg Al³⁺组与对照组相比差异无统计学意义（P>0.05），且5mg/kg和10mg/kg Al³⁺组海马细胞凋亡率与对照组相比显著降低（P<0.05）。各铝处理组bcl-2 mRNA表达与对照组相比均显著降低（P<0.05）。各铝处理组bax mRNA表达与对照组相比均显著升高（P<0.05）。铝与Bcl-2表达呈负相关（r=-0.909，P<0.01）。铝与Bax表达呈正相关（r=0.871，P<0.01）。
铝可通过下调bcl-2 mRNA、上调bax mRNA表达诱导大鼠海马细胞凋亡。
