State Key Laboratory of Pollution Control and Resource Reuse, College of Environmental Science and Engineering, Tongji University, Shanghai 200092, China; Shanghai Institute of Pollution Control and Ecological Security, Shanghai 200092, China.
State Key Laboratory of Pollution Control and Resource Reuse, College of Environmental Science and Engineering, Tongji University, Shanghai 200092, China.
Water Res. 2018 Jun 1;136:160-168. doi: 10.1016/j.watres.2018.02.031. Epub 2018 Feb 16.
Secondary water supply systems (SWSSs) refer to the in-building infrastructures (e.g., water storage tanks) used to supply water pressure beyond the main distribution systems. The purpose of this study was to investigate the influence of SWSSs on microbial community structure and the occurrence of opportunistic pathogens, the latter of which are an emerging public health concern. Higher numbers of bacterial 16S rRNA genes, Legionella and mycobacterial gene markers were found in public building taps served by SWSSs relative to the mains, regardless of the flushing practice (P < 0.05). In residential buildings, genes of L. pneumomhila, Acanthamoeba and Vermamoeba vermiformis were primarily detected in tanks and taps compared to the mains. Long water retention time, warm temperature and loss of disinfectant residuals promoted microbial growth and colonization of potential pathogens in SWSSs. Varied levels of microbial community shifts were found in different types of SWSSs during water transportation from the distribution main to taps, highlighting the critical role of SWSSs in shaping the drinking water microbiota. Overall, the results provided insight to factors that might aid in controlling pathogen proliferation in real-world water systems using SWSSs.
二次供水系统 (SWSS) 是指用于提供主分配系统以外的水压的建筑物内基础设施（例如，储水罐）。本研究旨在调查 SWSS 对微生物群落结构和机会性病原体发生的影响，后者是一个新出现的公共卫生关注点。无论冲洗实践如何（P<0.05），与主供水管相比，SWSS 服务的公共建筑龙头中发现了更多的细菌 16S rRNA 基因、军团菌和分枝杆菌基因标记。在住宅建筑中，与主供水管相比，L. pneumomhila、棘阿米巴和变形虫属的基因主要在水箱和龙头中检测到。长时间保持水温和失去消毒剂残留会促进 SWSS 中潜在病原体的微生物生长和定植。在从分配主管道到龙头的水输送过程中，不同类型的 SWSS 中发现了不同程度的微生物群落变化，突出了 SWSS 在塑造饮用水微生物组方面的关键作用。总体而言，这些结果提供了有关因素的见解，这些因素可能有助于控制实际用水系统中 SWSS 中病原体的增殖。
