Obstetrics and Gynaecology, Department of Clinical and Experimental Medicine, Linköping University, SE-58185, Linköping, Sweden; Department of Obstetrics & Gynaecology, County Hospital Sundsvall, SE-85643, Sundsvall, Sweden.
Department of Clinical and Experimental Medicine, Linköping University, SE-58185, Linköping, Sweden.
J Reprod Immunol. 2018 Apr;126:53-59. doi: 10.1016/j.jri.2018.02.006. Epub 2018 Mar 2.
To prospectively study systemic in vivo immunological effects of sex hormones, using different phases of oral combined hormonal contraceptives (CHC), and the natural menstrual cycles in both healthy women and in women with multiple sclerosis (MS), blood samples from sixty female MS patients and healthy controls with and without CHC were drawn in high and low estrogenic/progestogenic phases. Expression of Th-associated genes in blood cells was determined by qPCR and a panel of cytokines and chemokines was measured in plasma. High hormone level phases were associated with increases in Th1 (TBX21) and Th2 (GATA3) associated markers, as well as the B cell-associated chemokine CXCL13, while the inhibitory regulator CTLA-4 was decreased. These changes were not observed in MS patients, of whom most were treated with immunomodulatory drugs. Our data indicate immune activating properties in vivo of high steroid sex hormone levels during both CHC and normal menstrual cyclicity.
为了前瞻性研究性激素的全身体内免疫效应，使用不同阶段的口服复方激素避孕药（CHC）和健康女性以及多发性硬化症（MS）女性的自然月经周期，从 60 名患有 MS 的女性患者和使用和未使用 CHC 的健康对照者的血液样本中提取了高雌激素/孕激素阶段和低雌激素/孕激素阶段。通过 qPCR 测定血细胞中 Th 相关基因的表达，并在血浆中测定一组细胞因子和趋化因子。高激素水平阶段与 Th1（TBX21）和 Th2（GATA3）相关标志物的增加以及 B 细胞相关趋化因子 CXCL13 有关，而抑制调节因子 CTLA-4 则减少。这些变化在大多数接受免疫调节药物治疗的 MS 患者中并未观察到。我们的数据表明，在 CHC 和正常月经周期期间，高类固醇性激素水平具有体内免疫激活特性。
