Key Laboratory for Solid Waste Management and Environment Safety, Ministry of Education of China, School of Environment, Tsinghua University, Beijing 10084, PR China; College of Environmental Science and Engineering, Taiyuan University of Technology, Taiyuan 030024, PR China.
Key Laboratory for Solid Waste Management and Environment Safety, Ministry of Education of China, School of Environment, Tsinghua University, Beijing 10084, PR China.
Bioresour Technol. 2018 Jun;257:181-191. doi: 10.1016/j.biortech.2018.02.088. Epub 2018 Feb 23.
The impacts of ZVI on microbial community diversity in an anaerobic assay with high organic loading were investigated. The relative abundance of bacteria, archaea, and the functional methyl coenzyme-M reductase (mcrA) gene were investigated using high-throughput sequencing, and variations in their quantity were determined by qPCR. The results showed that ZVI significantly increased both the relative abundance and quantity of Methanobacteriales and Methanosarcinales during hydrogenotrophic and acetoclastic methanogenesis. The relative abundance of syntrophic Methanobacteriales at the hydrolysis and acidogenesis stages resulted in H partial pressure decrease through an interspecies hydrogen transfer (IHT) network, which further induced butyric conversion to acetic by Syntrophomonas. The primary microbial metabolism then converted to acetoclastic methanogensis in the assay with ZVI addition. The short duration of this process and high relative abundance of Syntrophomonas, Clostridium butyricum and Methanosarcinales potentially indicated the existence of a novelty syntrophic mechanism for extracellular electron transfer, which promoted CH generation.
研究了零价铁（ZVI）对高有机负荷厌氧试验中微生物群落多样性的影响。采用高通量测序技术研究了细菌、古菌和功能甲基辅酶-M 还原酶（mcrA）基因的相对丰度，并通过 qPCR 确定了它们的数量变化。结果表明，ZVI 显著增加了氢营养型和乙酰营养型产甲烷过程中甲烷杆菌目和甲烷八叠球菌目的相对丰度和数量。水解和产酸阶段的共营养甲烷杆菌目的相对丰度通过种间氢转移（IHT）网络导致 H 分压下降，从而进一步诱导丁酸转化为乙酸。在添加 ZVI 的试验中，主要微生物代谢随后转化为乙酰营养型产甲烷。这一过程的持续时间短，且共营养菌（Syntrophomonas）、丁酸梭菌和甲烷八叠球菌的相对丰度高，这可能表明存在一种新颖的细胞外电子传递共营养机制，促进了 CH 的生成。
