Frade R
Mol Immunol. 1986 Nov;23(11):1249-53. doi: 10.1016/0161-5890(86)90159-8.
Analysis of the interaction of human C3 fragments with human B lymphoma cell line, led us to isolate gp 140, the C3 receptor of Raji cells. Rabbit anti-gp 140 was prepared against this highly purified receptor. Using these polyclonal antibodies, it was found that: gp 140 is the C3d receptor (CR2) which reacts with the C3d site expressed on C3d, C3dg, C3bi and at a less extent on C3b. Gp 140 is a specific marker of human B lymphocytes; gp 140 is also the Epstein-Barr virus receptor (EBVR); CR2 is a membrane site involved in B-cell regulation; and the C3d/C3dg receptor (CR2) of human B lymphocytes is distinct to the C3dg receptor (CR4) of human neutrophils.
对人C3片段与人类B淋巴瘤细胞系相互作用的分析，使我们分离出了Raji细胞的C3受体gp 140。针对这种高度纯化的受体制备了兔抗gp 140。使用这些多克隆抗体，发现：gp 140是C3d受体（CR2），它与C3d、C3dg、C3bi上表达的C3d位点反应，与C3b的反应程度较低。Gp 140是人类B淋巴细胞的特异性标志物；gp 140也是爱泼斯坦-巴尔病毒受体（EBVR）；CR2是参与B细胞调节的膜位点；人类B淋巴细胞的C3d/C3dg受体（CR2）与人类中性粒细胞的C3dg受体（CR4）不同。
