Naka Shuhei, Wato Kaoruko, Hatakeyama Rina, Okawa Rena, Nomura Ryota, Nakano Kazuhiko
Department of Pediatric Dentistry, Osaka University Graduate School of Dentistry, Suita, Japan.
J Oral Microbiol. 2018 Jan 22;10(1):1428005. doi: 10.1080/20002297.2018.1428005. eCollection 2018.
: We previously reported that intravenous administration of strain TW871 caused typical non-alcoholic steatohepatitis (NASH)-like findings in a high-fat diet (HFD) mouse model at 16 weeks after initiating the experiment. : The purpose of the present study was to analyse mice administered TW871 fed a HFD for various periods of time. : First, 6-week-old C57BL/6J mice were fed an HFD for 4 weeks, then TW871 (1 × 10 CFU) or phosphate-buffered saline (PBS) were intravenously administered. Mice were euthanized 12, 16, 20, and 48 weeks after starting the experiment, and conventional clinical and histopathological evaluations were performed. : Typical NASH-like findings were not identified in the mice at 12 weeks, while they were observed in the TW871 group at 16 weeks, and the severity of NASH symptoms were increased at 20 weeks. Furthermore, signs of severe NASH were also observed at 48 weeks. In contrast, in the PBS-administered group, the NASH findings were identified only at 48 weeks and no typical NASH features were observed at 12, 16, or 20 weeks. : These results suggest that intravenous administration of a specific strain aggravates NASH in a time-dependent manner in the mice in contrast to mice without exposure.
我们之前报道过，在开始实验16周后，对高脂饮食（HFD）小鼠模型静脉注射TW871菌株会导致典型的非酒精性脂肪性肝炎（NASH）样表现。本研究的目的是分析在不同时间段喂食HFD并注射TW871的小鼠。首先，给6周龄的C57BL/6J小鼠喂食HFD 4周，然后静脉注射TW871（1×10⁸CFU）或磷酸盐缓冲盐水（PBS）。在开始实验后的12、16、20和48周对小鼠实施安乐死，并进行常规临床和组织病理学评估。在12周时未在小鼠中发现典型的NASH样表现，而在16周时在TW871组中观察到了这些表现，并且在20周时NASH症状的严重程度增加。此外，在48周时也观察到了严重NASH的迹象。相比之下，在注射PBS的组中，仅在48周时发现了NASH表现，而在12、16或20周时未观察到典型的NASH特征。这些结果表明，与未接触该菌株的小鼠相比，静脉注射特定菌株会使小鼠的NASH以时间依赖性方式加重。
