Clavel S, Garabedian M, Tau C, Orgiazzi J
Presse Med. 1987 Jan 31;16(3):107-10.
Mean plasma calcitriol was significantly increased in a patient with sarcoidosis and hypercalcemia without elevation of PTH. Recent studies provided evidence for an extrarenal production of calcitriol. To investigate this possibility, the conversion of calcidiol by a sarcoid lymph node homogenate was studied. After 2-hour incubation, a product was present in the incubation, which comigrated with synthetic calcitriol on two high performance liquid chromatography systems, was detected by ultraviolet absorption spectrometry and was bound with high affinity by the chick intestinal receptor for calcitriol. These results provide further evidence for an extrarenal synthesis of calcitriol, contributing to the excessive amounts of this metabolite found in the plasma of patients with sarcoidosis.
一名患有结节病和高钙血症且甲状旁腺激素未升高的患者，其血浆骨化三醇均值显著升高。近期研究为骨化三醇的肾外生成提供了证据。为研究这种可能性，对结节病淋巴结匀浆将骨化二醇转化的情况进行了研究。孵育2小时后，孵育物中出现了一种产物，该产物在两种高效液相色谱系统上与合成骨化三醇一同迁移，通过紫外吸收光谱法检测到，并与鸡肠道骨化三醇受体具有高亲和力结合。这些结果为骨化三醇的肾外合成提供了进一步证据，这导致结节病患者血浆中发现的这种代谢产物过量。
