结节病肉芽肿在体外可代谢25-羟基维生素D3。

Sarcoid granulomas metabolize 25-hydroxyvitamin D3 in vitro.

作者信息

Okabe T, Ishizuka S, Fujisawa M, Watanabe J, Takaku F

出版信息

Biochem Biophys Res Commun. 1984 Sep 17;123(2):822-30. doi: 10.1016/0006-291x(84)90304-8.

Abstract

Sarcoid granulomas metabolized 25-hydroxyvitamin D3 to two unidentified metabolites during in vitro incubation. A two-step high pressure liquid chromatography system revealed two unique elution positions of these sarcoid-derived metabolites that exactly comigrated with the elution positions of 5(Z)-19-nor-10-oxo-25(OH)D3 and 5(E)-19-nor-10-oxo-25(OH)D3, respectively. These unique metabolites did not bind specifically to a protein receptor for 1,25(OH)2D3.

摘要

在体外培养过程中，结节病肉芽肿将25-羟基维生素D3代谢为两种未鉴定的代谢产物。两步高压液相色谱系统显示，这些结节病衍生代谢产物有两个独特的洗脱位置，分别与5(Z)-19-去甲-10-氧代-25(OH)D3和5(E)-19-去甲-10-氧代-25(OH)D3的洗脱位置完全一致。这些独特的代谢产物不会特异性结合1,25(OH)2D3的蛋白受体。