Hafiz I, Bhatti H N, Hanif M A, Shahid M
Department of Chemistry, University of Agriculture, Faisalabad, Pakistan.
Department of Biochemistry, University of Agriculture, Faisalabad, Pakistan.
J Biol Regul Homeost Agents. 2018 Jan-Feb;32(1):101-106.
Herbs and plants are mostly used as antimicrobials and antioxidants owing to the harmfulness and linked side-effects of synthetic chemical constituents. Plants and spices produce various metabolites with antibacterial and antioxidant potential. These metabolites are principally revealed as encouraging healing components or mediators which control ailments in human beings. The present study was aimed to characterize the extracts from selected medicinal plants through in-vitro activities. Winged prickly ash, green tea and thyme were selected and extracted through ethanol and methanol solutions. The extracts were assessed for antibacterial and antioxidant activities. The antibacterial potential of extracts showed the significant extent of the activity against Bacillus subtilis and E. coli. The maximum activity was noted in 80% methanolic fraction of Thymus vulgaris (15.20±0.64 mm) against Bacillus subtilis. Antioxidant potential exhibited the highest phenolic and flavonoid content in Camellia sinensis . The total phenolic content was significantly higher (1456.26±12.05 mg gallic acid) in 80% ethanolic fraction of Camellia sinensis. The flavonoid content in different plant extracts ranged from 8.17±2.02 to 376.29±7.11 mg/g. The radical scavenging DPPH assay also showed the significant antioxidant capacity of selected plants with the methanolic (50%) extract of Camellia sinensis found to be the most potent (78.95±7.12%). It was concluded that the alcoholic extracts of selected medicinal plants revealed the effective antibacterial and antioxidant activity, showing protective prospective against oxidative injury.
由于合成化学成分的有害性及其相关副作用,草药和植物大多被用作抗菌剂和抗氧化剂。植物和香料会产生具有抗菌和抗氧化潜力的各种代谢物。这些代谢物主要被视为促进愈合的成分或介质,可控制人类疾病。本研究旨在通过体外活性对选定药用植物的提取物进行表征。选取了花椒、绿茶和百里香,并通过乙醇和甲醇溶液进行提取。对提取物的抗菌和抗氧化活性进行了评估。提取物的抗菌潜力显示出对枯草芽孢杆菌和大肠杆菌具有显著的活性程度。在百里香的80%甲醇提取物对枯草芽孢杆菌的作用中观察到最大活性(15.20±0.64毫米)。抗氧化潜力方面,茶树中酚类和黄酮类含量最高。茶树80%乙醇提取物中的总酚含量显著更高(1456.26±12.05毫克没食子酸)。不同植物提取物中的黄酮类含量在8.17±2.02至376.29±7.11毫克/克之间。自由基清除DPPH试验也显示选定植物具有显著的抗氧化能力,其中茶树的50%甲醇提取物最为有效(78.95±7.12%)。得出的结论是,选定药用植物的醇提取物显示出有效的抗菌和抗氧化活性,对氧化损伤具有潜在的保护作用。