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对[具体物质]的乙醇提取物和甲醇提取物的抗菌、抗氧化、溶血和溶栓活性进行研究。 (你提供的原文中“ , , and ”部分内容缺失,请补充完整以便更准确翻译)

Investigation of the antimicrobial, antioxidant, hemolytic, and thrombolytic activities of , , and ethanolic and methanolic extracts.

作者信息

Rafique Sobia, Murtaza Mian Anjum, Hafiz Iram, Ameer Kashif, Qayyum Mir Muhammad Nasir, Yaqub Shazia, Mohamed Ahmed Isam A

机构信息

Institute of Food Science and Nutrition University of Sargodha Sargodha Pakistan.

Institute of Chemistry University of Sargodha Sargodha Pakistan.

出版信息

Food Sci Nutr. 2023 Jul 24;11(10):6303-6311. doi: 10.1002/fsn3.3569. eCollection 2023 Oct.

Abstract

is rich in antioxidants such as polyphenols; contains bioactive compounds (flavonoids, terpenoids, and tannins) and is primarily composed of volatile oils, amides, alkaloids, flavonoids, lignan, and coumarin. The antibacterial, antifungal, biofilm inhibition, antioxidant, hemolytic, and thrombolytic activities of , , and ethanol and methanol extracts at different concentrations (30%, 50%, and 80%) were determined. The antioxidant activity and content were measured as free radical scavenging assay (DPPH), total flavonoid content (TFC), and total phenolic content (TPC). Furthermore, hemolytic and thrombolytic analysis was carried out to determine toxicity. In antimicrobial assays, 80% methanol thyme extract showed highest (15.31 mm) antibacterial activity against , and 80% ethanol green tea extract showed optimal antibacterial activity against . Ethanol 30% green tea extract resulted in highest (26.61 mm) antifungal activity against . The maximum (54.73%) biofilm inhibition was resulted by methanol 50% thyme extract for . In antioxidant activity and content, methanol 50% green tea extract had highest (80.82%) antioxidant activity, whereas, ethanol 80% green tea extract had maximum (1474.55 mg CE/g DW) TFC and methanol 80% green tea extract had maximum (593.05 mg GAE/g) TPC. In toxicological assays, methanol 30% green tea extract had highest (25.28%) thrombolytic activity, and ethanol 80% tejphal extract had maximum (18.24%) hemolytic activity. This study has highlighted the significant antimicrobial, antioxidant, hemolytic, and thrombolytic activities of , , and extracts that could be beneficial to treat various diseases (cancer, diabetes, and respiratory diseases) and may be utilized as functional ingredient in the preparation of functional foods and drinks.

摘要

富含多酚等抗氧化剂;含有生物活性化合物(黄酮类、萜类和单宁),主要由挥发油、酰胺、生物碱、黄酮类、木脂素和香豆素组成。测定了不同浓度(30%、50%和80%)的[具体物质1]、[具体物质2]和[具体物质3]乙醇和甲醇提取物的抗菌、抗真菌、生物膜抑制、抗氧化、溶血和溶栓活性。抗氧化活性和含量通过自由基清除试验(DPPH)、总黄酮含量(TFC)和总酚含量(TPC)进行测定。此外,进行溶血和溶栓分析以确定毒性。在抗菌试验中,80%甲醇百里香提取物对[某种细菌]显示出最高(15.31毫米)的抗菌活性,80%乙醇绿茶提取物对[另一种细菌]显示出最佳抗菌活性。30%乙醇绿茶提取物对[某种真菌]产生了最高(26.61毫米)的抗真菌活性。50%甲醇百里香提取物对[具体对象]产生了最大(54.73%)的生物膜抑制作用。在抗氧化活性和含量方面,50%甲醇绿茶提取物具有最高(80.82%)的抗氧化活性,而80%乙醇绿茶提取物具有最大(1474.55毫克CE/克干重)的TFC,80%甲醇绿茶提取物具有最大(593.05毫克GAE/克)的TPC。在毒理学试验中,30%甲醇绿茶提取物具有最高(25.28%)的溶栓活性,80%乙醇tejphal提取物具有最大(18.24%)的溶血活性。本研究突出了[具体物质1]、[具体物质2]和[具体物质3]提取物显著的抗菌、抗氧化、溶血和溶栓活性,这些活性可能有益于治疗各种疾病(癌症、糖尿病和呼吸道疾病),并可用作功能性食品和饮料制备中的功能性成分。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/3a1d/10563746/c6256941783c/FSN3-11-6303-g001.jpg

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