Department of Hematology and Oncology, Inner Mongolia Forestry General Hospital, Yakeshi 022150, China.
Department of Thoracic Surgery, Beijing Chest Hospital, Capital Medical University, Beijing 101149, China.
Int J Biol Macromol. 2018 Jul 1;113:849-858. doi: 10.1016/j.ijbiomac.2018.03.005. Epub 2018 Mar 2.
In present study, we purified a polysaccharide, TFPB1, from the flower buds of Tussilago farfara using DEAE-cellulose 52 anion-exchange and Sephacryl S-300 HR gel filtration chromatography. TFPB1 was a homogeneous polysaccharide with a molecular weight of 37.8kDa and composed of rhamnose, galacturonic acid, glucose, galactose, and arabinose, in a ratio of 13:13:1:7:12. Methylation and NMR results demonstrated that TFPB1 contained a rhamnogalacturonan I backbone consisting of a repeat disaccharide unit →4)-α--GalAp-(1→2)-α--Rhap-(1→, substituted by various type II arabinogalactan branches including terminal galactose, (1→3)-β--galactan and (1→5)-α--arabinan, attached to the O-4 of (1→2)-α--Rhap. TFPB1 was found to inhibit cell proliferation of A549 cells and induce cell apoptosis in vitro. Furthermore, TFPB1 downregulated the phosphorylation of Akt, and upregulated caspase-3, Fas, FasL, and Bax expression, but downregulated Bcl-2 expression. Therefore, TFPB1 exhibited anti-proliferative and anti-apoptotic effect partly depending on the suppression of Akt signaling pathway. These findings provided us a potential chemotherapeutic strategy for the treatment of human non-small cell lung cancer.
在本研究中，我们使用 DEAE-纤维素 52 阴离子交换和 Sephacryl S-300 HR 凝胶过滤层析从款冬花的花蕾中纯化出一种多糖，TFPB1。TFPB1 是一种均一多糖，分子量为 37.8kDa，由鼠李糖、半乳糖醛酸、葡萄糖、半乳糖和阿拉伯糖组成，摩尔比为 13:13:1:7:12。甲基化和 NMR 结果表明，TFPB1 含有一个由重复二糖单元→4)-α--GalAp-(1→2)-α--Rhap-(1→组成的鼠李半乳糖醛酸聚糖 I 骨架，由各种类型的 II 阿拉伯半乳聚糖支链取代，包括末端半乳糖、(1→3)-β--半乳糖和(1→5)-α--阿拉伯聚糖，连接到(1→2)-α--Rhap 的 O-4 上。TFPB1 被发现能抑制 A549 细胞的增殖并诱导细胞凋亡。此外，TFPB1 下调 Akt 的磷酸化，上调 caspase-3、Fas、FasL 和 Bax 的表达，但下调 Bcl-2 的表达。因此，TFPB1 表现出抗增殖和抗凋亡作用，部分依赖于 Akt 信号通路的抑制。这些发现为治疗人类非小细胞肺癌提供了一种潜在的化疗策略。
