Department of Fragrance and Cosmetic Science, Kaohsiung Medical University, Kaohsiung 807, Taiwan.
School of Medical and Health Sciences, Fooyin University, Kaohsiung 831, Taiwan.
Oxid Med Cell Longev. 2018 Feb 4;2018:6354972. doi: 10.1155/2018/6354972. eCollection 2018.
-Hydroxycinnamoylphenalkylamides (36H) exhibited both antioxidation and antityrosinase abilities. The compound was studied for its antioxidative properties, using a 1,1-diphenyl-2-picrylhydrazul- (DPPH-) scavenging test, a ferric ion-reducing antioxidant power assay (FRAP) assessment, and a metal-chelating power assay. The results showed that 36H had antioxidative capabilities in the DPPH-scavenging and ferric-reducing power examinations but the chelating power assay did not demonstrate antioxidative capability. 36H was also measured for tyrosinase inhibitory activity applying various species platforms, including in vitro mushroom, B16F10 mouse melanoma, and human melanocyte cells. In terms of in vitro mushroom tyrosinase suppression, 36H restrained the melanogenesis processes. It is assumed that 36H blocked the tyrosinase active site as a competitive inhibitor for mushroom tyrosinase, hence not decreasing the human normal melanocyte cellular viability. A quantitative real-time polymerase chain reaction (qRT-PCR) and western blot discovered that 36H downregulated melanogenesis-related RNA and proteins, including pigment production (MITF, tyrosinase, TRP-1, and TRP-2), melanosome maturation (Rab27a), and melanosome transportation (Myo5a, MLPH and Mreg). Overall, 36H displayed the biofunctions of antioxidation and melanin suppression, so there was a possibility for its application as a food additive or a skin-whitening agent.
羟基肉桂酰苯烷基酰胺（36H）具有抗氧化和酪氨酸酶抑制活性。该化合物的抗氧化性能通过 1,1-二苯基-2-苦基肼基（DPPH-）清除试验、铁离子还原抗氧化能力（FRAP）评估和金属螯合能力测定进行研究。结果表明，36H 在 DPPH 清除和铁还原能力试验中具有抗氧化能力，但螯合能力测定没有显示抗氧化能力。36H 还通过各种物种平台测量了对酪氨酸酶抑制活性的影响，包括体外蘑菇、B16F10 小鼠黑色素瘤和人黑素细胞。在体外蘑菇酪氨酸酶抑制方面，36H 抑制了黑色素生成过程。据推测，36H 作为竞争性抑制剂阻断了蘑菇酪氨酸酶的活性位点，因此不会降低人正常黑素细胞的活力。实时定量聚合酶链反应（qRT-PCR）和 Western blot 发现，36H 下调了黑色素生成相关的 RNA 和蛋白质，包括色素生成（MITF、酪氨酸酶、TRP-1 和 TRP-2）、黑素体成熟（Rab27a）和黑素体运输（Myo5a、MLPH 和 Mreg）。总体而言，36H 显示出抗氧化和黑色素抑制的生物功能，因此有可能作为食品添加剂或皮肤美白剂应用。
