Institute of Biotechnology, National Taiwan University, Taipei, Taiwan.
Livestock Research Institute, Council of Agriculture, Executive Yuan, Tainan, Taiwan.
Mol Nutr Food Res. 2018 Apr;62(8):e1700903. doi: 10.1002/mnfr.201700903. Epub 2018 Apr 3.
Non-alcoholic fatty liver disease (NAFLD) is a common disease that is concomitant with obesity, resulting in increased mortality. To date, the efficiency of NAFLD treatment still needs to be improved. Therefore, we aimed to evaluate the effect of Lactobacillus mali APS1, which was isolated from sugary kefir, on hepatic steatosis in rats fed a high-fat diet (HFD).
Sprague Dawley rats were fed a control diet, a HFD with saline, and a HFD with APS1 intervention by gavage daily for 12 weeks. The results showed that APS1 significantly reduced body weight and body weight gain in HFD-fed rats. APS1 reduced hepatic lipid accumulation by regulating SIRT-1/PGC-1α/SREBP-1 expression. Moreover, APS1 increased hepatic antioxidant activity by modulating Nrf-2/HO-1 expression. Notably, APS1 manipulated the gut microbiota, resulting in increasing proportions of the phylum Bacteroidetes/Firmicutes and reducing the abundance of specific NAFLD-associated bacteria.
These results suggested that APS1 ameliorated hepatic steatosis by modulating lipid metabolism and antioxidant activity via manipulating specific NAFLD-associated gut microbiota in vivo.
非酒精性脂肪性肝病（NAFLD）是一种常见的疾病，与肥胖并存，导致死亡率增加。迄今为止，NAFLD 的治疗效果仍有待提高。因此，我们旨在评估从含糖开菲尔中分离出的苹果酸乳杆菌 APS1 对高脂肪饮食（HFD）喂养大鼠肝脂肪变性的影响。
Sprague Dawley 大鼠分别用对照饲料、HFD 加盐水、HFD 加 APS1 灌胃喂养 12 周。结果表明，APS1 可显著降低 HFD 喂养大鼠的体重和体重增加。APS1 通过调节 SIRT-1/PGC-1α/SREBP-1 表达来减少肝脂质积累。此外，APS1 通过调节 Nrf-2/HO-1 表达来增加肝抗氧化活性。值得注意的是，APS1 操纵肠道微生物群，导致厚壁菌门/Firmicutes 的比例增加，并减少特定与 NAFLD 相关的细菌的丰度。
这些结果表明，APS1 通过调节与 NAFLD 相关的特定肠道微生物群，在体内改善了脂质代谢和抗氧化活性，从而改善了肝脂肪变性。
