Firsova Yu E, Torgonskaya M L, Trotsenko Yu A
Prikl Biokhim Mikrobiol. 2017 Mar-Apr;53(2):196-203.
A knockout mutant of Methylobacterium dichloromethanicum DM4 with an inactivated gene of a putative transcription regulator METDI5511 (ΔMETDI5511) has been obtained. The expression of this gene increases many times when the strain is grown on dichloromethane compared to methanol. The mutant had a low growth rate on dichloromethane as compared with the original strain and was found to be more sensitive to influences of various types of stress (oxidative, osmotic stress, heat, and drying). The cells were stained with Fluorescent Brightener 28 (Calcofluor white), and the intensity of their fluorescence showed that the ΔMETDI5511 mutant had significantly increased numbers of surface polysaccharides with β-1,3 and β-1,4-glycoside bonds. The results indicate that the METDI5511 gene is involved in the regulation of surface polysaccharides that play an important role in adaptation of cells to growth on dichloromethane.
已获得二氯甲烷甲基杆菌DM4的基因敲除突变体，其假定转录调节因子METDI5511的基因被灭活（ΔMETDI5511）。与在甲醇上生长相比，当该菌株在二氯甲烷上生长时，该基因的表达增加了许多倍。与原始菌株相比，该突变体在二氯甲烷上的生长速率较低，并且发现其对各种类型的应激（氧化应激、渗透应激、热应激和干燥）的影响更敏感。用荧光增白剂28（荧光增白剂）对细胞进行染色，其荧光强度表明，ΔMETDI5511突变体具有β-1,3和β-1,4糖苷键的表面多糖数量显著增加。结果表明，METDI5511基因参与表面多糖的调节，表面多糖在细胞适应二氯甲烷生长中起重要作用。
