Firsova Yu E, Torgonskaya M L, Trotsenko Yu A
Mikrobiologiia. 2015 Nov-Dec;84(6):697-704.
Activation of expression of the xoxFgene encoding PQQ-dependent methanol/ethanol dehydrogenase (METDI2492) in dichloromethane- (DCM) -grown Methylobacterium dichloromethanicum DM4 was first demonstrated. The sequence of the only XoxF homolog found in the genome of strain DM4 exhibited 50% identity to that of the protein (MxaF) of the large subunit of methanol dehydrogenase (MDH). A knockout mutant with the inactivate xoxF gene (ΔxoxF) was found to be unable to grow on methanol due to the absence of the expression of the gene cluster of the classical MDH, as was confirmed by the GFP test. When grown of succinate, the ΔxoxF mutant exhibited a lower growth rate on DCM than the original strain and was more sensitive to various stress factors (oxidative, osmotic, and heat shock). Based on these data, the xoxF gene was hypothesized to belong to a group of genes affecting expression of the proteins of general stress response.
首次证明，在二氯甲烷（DCM）中生长的 Dichloromethanicum 甲基杆菌 DM4 中，编码 PQQ 依赖性甲醇/乙醇脱氢酶（METDI2492）的 xoxF 基因的表达被激活。在菌株 DM4 的基因组中发现的唯一 XoxF 同源物的序列与甲醇脱氢酶（MDH）大亚基的蛋白质（MxaF）的序列具有 50% 的同一性。通过 GFP 测试证实，由于经典 MDH 基因簇的表达缺失，具有失活 xoxF 基因（ΔxoxF）的敲除突变体无法在甲醇上生长。当在琥珀酸盐上生长时，ΔxoxF 突变体在 DCM 上的生长速率低于原始菌株，并且对各种应激因素（氧化、渗透和热休克）更敏感。基于这些数据，推测 xoxF 基因属于影响一般应激反应蛋白质表达的一组基因。
