Department of Neurology, Xianya Hospital of Central South University, Xianya, Hunan Province, China.
Eur Rev Med Pharmacol Sci. 2018 Feb;22(4):1078-1083. doi: 10.26355/eurrev_201802_14394.
The present study was designed to investigate the effect of microRNA-126 (miR-126) on the migration and homing of endothelial progenitor cells (EPCs) within arterial thrombus of cerebral infarction patients.
EPCs from rat bone marrow were isolated, and miR-126 overexpressed EPCs were constructed by lentiviral transfection. Then, the middle cerebral artery occlusion (MCAO) model was established by the method of thread ligation. Successfully established model rats were randomly divided into miR-126 overexpression EPC group, miR-126 wild type EPC group, and normal saline group. One day after the infarction, the miR-126 overexpression EPCs, miR-126 wild type EPCs, and normal saline, were injected into the lateral ventricle of the corresponding groups. Also, the transplanted cells were tracked by cell dye SPDiIC18. The expression of tight junction proteins ZO-1 and Claudin-5 in brain tissue was detected by Western blotting.
Transplanted cells were detected in the cerebral infarction area 3 days after transplantation by cell dye SP-DiIC18. The number of homing EPCs in miR-126 overexpression group was significantly higher than that of miR-126 wild type EPC group (p < 0.05). Also, the protein expression of ZO-1 and Claudin-5 in the miR- 126 overexpression EPC group was significantly higher compared with that of the miR-126 wild type EPC group and the normal saline group.
miR-126 overexpression EPCs, which were transplanted in the lateral ventricle, can home to the cerebral infarction areas via increasing increase.
本研究旨在探讨 microRNA-126（miR-126）对脑梗死患者动脉血栓内内皮祖细胞（EPC）迁移和归巢的影响。
分离大鼠骨髓来源的 EPC，通过慢病毒转染构建 miR-126 过表达 EPC。然后，采用线栓法建立大脑中动脉闭塞（MCAO）模型。成功建立模型的大鼠随机分为 miR-126 过表达 EPC 组、miR-126 野生型 EPC 组和生理盐水组。在梗死 1 天后，将 miR-126 过表达 EPC、miR-126 野生型 EPC 和生理盐水分别注入相应组的侧脑室。同时，用细胞染料 SPDiIC18 追踪移植细胞。通过 Western blot 检测脑组织中紧密连接蛋白 ZO-1 和 Claudin-5 的表达。
通过细胞染料 SP-DiIC18 检测到移植细胞在脑梗死区域 3 天后。miR-126 过表达组归巢 EPC 数量明显高于 miR-126 野生型 EPC 组（p<0.05）。此外，miR-126 过表达 EPC 组的 ZO-1 和 Claudin-5 蛋白表达明显高于 miR-126 野生型 EPC 组和生理盐水组。
转染到侧脑室的 miR-126 过表达 EPC 可以通过增加归巢来归巢到脑梗死区域。
