Imanishi J, Tanaka A, Matsuoka H, Kakui Y, Uemura H, Imanishi T, Nishino H
C R Seances Soc Biol Fil. 1986;180(5):596-600.
Human tumor-degenerating factor (TDF), which is produced in the culture of human fibroblasts, causes the degenerative changes of the tumor cells and forms a cell-free area on the surface of culture dish. Its degenerating activity is enhanced by the treatment with interferons. In the present study, it was recognized that TDF preparation inhibited the colony formation of KB cells according to the TDF activity. When recombinant human leukocyte interferon (rHuIFN-alpha A) was added to the KB cell culture, the colony formation was inhibited. The combination of TDF preparation with rHuIFN-alpha A inhibited more strongly the colony formation than TDF alone or rHuIFN-alpha A alone. It was found that the combination of TDF preparation with rHuIFN-gamma inhibited more remarkably the colony formation than the combination of TDF preparation with rHuIFN-alpha A.
人肿瘤退化因子（TDF）由人成纤维细胞培养产生，可引起肿瘤细胞的退化性变化，并在培养皿表面形成无细胞区域。用干扰素处理可增强其退化活性。在本研究中，根据TDF活性发现TDF制剂可抑制KB细胞的集落形成。当将重组人白细胞干扰素（rHuIFN-αA）添加到KB细胞培养物中时，集落形成受到抑制。TDF制剂与rHuIFN-αA联合使用比单独使用TDF或rHuIFN-αA更强烈地抑制集落形成。发现TDF制剂与rHuIFN-γ联合使用比TDF制剂与rHuIFN-αA联合使用更显著地抑制集落形成。
