Imanishi J, Matsuoka H, Hoshino S, Uemura H, Tanaka A, Imanishi T, Kishida T
C R Seances Soc Biol Fil. 1983;177(4):570-3.
The culture supernatant of human fibroblasts caused degenerative changes in the target cells. This tumor degenerating factor in the supernatant (TDF) appeared already on the 1st day of culture and increased gradually to the 8th day. TDF was effective on human KB, HeLa, FL and of hepatoma cells, but neither on murine L929, 3T3, SV-3T3 cells nor MDBK cells. Furthermore, TDF was not effective on human non-transformed cells, namely various human fibroblasts. Human leukocyte interferon (HuIFN-alpha) enhanced TDF production. The coculture of human fibroblasts with KB cells augmented TDF production.
人成纤维细胞的培养上清液可使靶细胞发生退行性变化。上清液中的这种肿瘤退化因子（TDF）在培养第1天就已出现，并逐渐增加至第8天。TDF对人KB、HeLa、FL及肝癌细胞有效，但对鼠L929、3T3、SV - 3T3细胞及MDBK细胞无效。此外，TDF对人未转化细胞，即各种人成纤维细胞也无效。人白细胞干扰素（HuIFN-α）可增强TDF的产生。人成纤维细胞与KB细胞共培养可增加TDF的产生。
