Adugna Shimeles, Kebede Tadesse, Mekonnen Zeleke, Degarege Abraham, Liang Song, Erko Berhanu
Addis Ababa University, College of Health Sciences, Department of Microbiology, Immunology and Parasitology, P.O. Box 1176, Addis Ababa.
Jimma University, School of Medical Laboratory Sciences, P.O. Box 378, Jimma.
Trans R Soc Trop Med Hyg. 2017 Dec 1;111(12):572-578. doi: 10.1093/trstmh/try010.
In this cross-sectional study, we compared the performance of Mini Parasep® solvent-free (SF) faecal parasite concentrator, Kato-Katz thick smear and McMaster techniques for the diagnosis of intestinal parasitic infections among children in Wosha Soyama Primary School, Ethiopia.
Stool samples were collected from 381 children and examined for intestinal parasitic infections using Mini Parasep® SF faecal parasite concentrator, Kato-Katz thick smear and McMaster techniques.
About 86.1% of children were infected with at least one species of intestinal parasite based on combined results of the three techniques. The sensitivity and negative predictive values of Mini Parasep® SF, Kato-Katz and McMaster tests for detecting at least one species of intestinal parasite infections were 90.2% and 62.4%, 80.0% and 44.5%, and 55.2% and 26.5%, respectively. While Mini Parasep® SF was more sensitive in detecting Ascaris lumbricoides, Schistosoma mansoni and Hymenolepis nana infections, Kato-Katz was more sensitive in detecting Trichuris trichiura infection, and McMaster had higher sensitivity in diagnosing hookworm infection.
The Mini Parasep® SF faecal parasite concentrator technique showed better performance than the Kato-Katz and McMaster techniques for the detection of intestinal helminth infections in stool samples, particularly for S. mansoni, A. lumbricoides and H. nana. Hence, Mini Parasep® SF could be used as one of the suitable faecal examination methods for surveillance and monitoring of preventive chemotherapy of schistosomiasis.
在这项横断面研究中，我们比较了Mini Parasep®无溶剂（SF）粪便寄生虫浓缩器、加藤厚涂片法和麦克马斯特技术在埃塞俄比亚沃沙索亚马小学儿童肠道寄生虫感染诊断中的性能。
从381名儿童中采集粪便样本，使用Mini Parasep® SF粪便寄生虫浓缩器、加藤厚涂片法和麦克马斯特技术检测肠道寄生虫感染。
根据这三种技术的综合结果，约86.1%的儿童感染了至少一种肠道寄生虫。Mini Parasep® SF、加藤厚涂片法和麦克马斯特检测法检测至少一种肠道寄生虫感染的敏感性和阴性预测值分别为90.2%和62.4%、80.0%和44.5%、55.2%和26.5%。虽然Mini Parasep® SF在检测蛔虫、曼氏血吸虫和微小膜壳绦虫感染方面更敏感，但加藤厚涂片法在检测鞭虫感染方面更敏感，而麦克马斯特法在诊断钩虫感染方面具有更高的敏感性。
Mini Parasep® SF粪便寄生虫浓缩器技术在检测粪便样本中的肠道蠕虫感染方面表现优于加藤厚涂片法和麦克马斯特技术，特别是对于曼氏血吸虫、蛔虫和微小膜壳绦虫。因此，Mini Parasep® SF可作为血吸虫病预防性化疗监测和监测的合适粪便检查方法之一。
