Centre for Research and Knowledge Transfer in Biotechnology, University of Zagreb, Zagreb, Croatia.
Center of Excellence for Viral Immunology and Vaccines, CERVirVac, Zagreb, Croatia.
Intervirology. 2017;60(5):181-189. doi: 10.1159/000487049. Epub 2018 Mar 6.
The families Paramyxoviridae and Pneumoviridae comprise a broad spectrum of viral pathogens that affect human health. The matrix (M) protein of these viruses has a central role in their life cycle. In line with this, molecular characteristics of the M proteins from variable viruses that circulated in Croatia were investigated.
Sequences of the M proteins of human parainfluenza virus (HPIV) 1-3 within the family Paramyxoviridae, human metapneumovirus (HMPV), and human respiratory syncytial virus from the family Pneumoviridae were obtained and analyzed.
M proteins were very diverse among HPIVs, but highly conserved within each virus. More variability was seen in nucleotide sequences of M proteins from the Pneumoviridae family. An insertion of 8 nucleotides in the 3' untranslated region in 1 HMPV M gene sequence was discovered (HR347-12). As there are no samples with such an insertion in the database, this insertion is of interest and requires further research.
While we have confirmed that M proteins were conserved among individual viruses, any changes that are observed should be given attention and further researched. Of special interest is inclusion of HPIV2 M proteins in this analysis, as these proteins have not been studied to the same extent as other paramyxoviruses.
副黏液病毒科和肺病毒科家族包含了广泛的影响人类健康的病毒病原体。这些病毒的基质（M）蛋白在其生命周期中起着核心作用。因此，对在克罗地亚流行的不同病毒的 M 蛋白的分子特征进行了研究。
获得了副黏液病毒科的人副流感病毒（HPIV）1-3、人偏肺病毒（HMPV）和肺病毒科的人呼吸道合胞病毒的 M 蛋白的序列，并进行了分析。
M 蛋白在 HPIV 之间差异很大，但在每种病毒内高度保守。在肺病毒科的 M 蛋白的核苷酸序列中观察到更多的变异。在 1 个 HMPV M 基因序列的 3'非翻译区发现了 8 个核苷酸的插入（HR347-12）。由于数据库中没有这种插入的样本，因此这种插入很有趣，需要进一步研究。
虽然我们已经证实了 M 蛋白在个体病毒之间是保守的，但任何观察到的变化都应该引起关注并进一步研究。特别值得关注的是将 HPIV2 M 蛋白纳入此分析，因为这些蛋白尚未像其他副黏液病毒那样进行过研究。
