Power U F, Ryan K W, Portner A
Department of Virology and Molecular Biology, St. Jude Children's Research Hospital, Memphis, Tennessee 38101.
Virology. 1992 Dec;191(2):947-52. doi: 10.1016/0042-6822(92)90270-y.
The nucleotide sequence of the M gene of human parainfluenza virus type 1 (hPIV1) was determined from genomic RNA and cDNA copies of the entire gene. The M gene contained 1173 nucleotides. It had one large open reading frame capable of encoding a protein of 348 amino acids (M(r) = 38,404). The predicted amino acid sequence of the hPIV1 M protein is highly basic (+20 at neutral pH). A pGEM-1 expression vector containing the M gene was used for cell-free transcription and translation. The resultant protein was confirmed to be M by electrophoretic mobility and immunoprecipitation. Among other paramyxoviridae the hPIV1 M amino acid sequence was most closely related to the Sendai virus M sequence (87% identity). The pattern of M gene relatedness observed from the alignment of 16 paramyxoviridae M protein amino acid sequences was not predicted by the viruses' taxonomic classification.
从人1型副流感病毒（hPIV1）的基因组RNA和整个基因的cDNA拷贝中测定了M基因的核苷酸序列。M基因包含1173个核苷酸。它有一个大的开放阅读框，能够编码一个由348个氨基酸组成的蛋白质（M(r)=38,404）。hPIV1 M蛋白的预测氨基酸序列具有高度碱性（在中性pH下为+20）。一个含有M基因的pGEM-1表达载体用于无细胞转录和翻译。通过电泳迁移率和免疫沉淀证实所得蛋白质为M。在其他副粘病毒科中，hPIV1 M氨基酸序列与仙台病毒M序列关系最为密切（同一性为87%）。从16种副粘病毒科M蛋白氨基酸序列比对中观察到的M基因相关性模式并未由病毒的分类学分类所预测。
