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来自（此处原文“(L.)”信息不完整，无法准确翻译）的葫芦素D、E和I上调基因并诱导人胃癌细胞系AGS中的细胞周期停滞。

The cucurbitacins D, E, and I from (L.) upregulate the gene and induce cell-cycle arrest in human gastric cancer cell line AGS.

作者信息

Jafargholizadeh Naser, Zargar Seyed Jalal, Aftabi Younes

机构信息

Department of Cell & Molecular Biology, School of Biology, College of Science, University of Tehran, Tehran, Iran.

Tuberculosis and Lung Disease Research Center, Tabriz University of Medical Sciences, Tabriz, Iran.

出版信息

Iran J Basic Med Sci. 2018 Mar;21(3):253-259. doi: 10.22038/ijbms.2018.25175.6236.

DOI:10.22038/ijbms.2018.25175.6236
PMID:29511491
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC5817168/
Abstract

OBJECTIVES

Cucurbitacins exhibit a range of anti-cancer functions. We investigated the effects of cucurbitacins D, E, and I purified from (L.) A. Rich fruits on some apoptotic and autophagy genes in human gastric cancer cell line AGS.

MATERIALS AND METHODS

Using quantitative reverse transcription PCR (qRT-PCR), the expression of , , , , and genes were quantified in AGS cells 24 hr after treatment with cucurbitacins D, E, and I at concentrations 0.3, 0.1 and 0.5 μg/ml, respectively. Cell cycle and death were analyzed by flflowcytometry.

RESULTS

Purified cucurbitacins induced sub-G1 cell-cycle arrest and cell death in AGS cells and upregulated mRNA effectively, but showed a very low effect on , , and mRNA levels. Also after treatment with cucurbitacin I at concentration 0.5 μg/ml, mRNA levels were increased about 4.4 times. Pairwise comparison of the effect of cucurbitacins D, E, and I on mRNA expression showed that the cucurbitacin I effect is 1.3 and 1.1 times that of cucurbitacins E and D, respectively; cucurbitacin D effect is 1.2 times that of cucurbitacin E ( <0.05). analysis showed that among autophagy genes, has an important gastric cancer rank relation.

CONCLUSION

Cucurbitacins D, E, and I purified from fruits upregulate and induce sub-G1 cell-cycle arrest and cell death in human gastric cancer cell line AGS. Cucurbitacin I effect on mRNA expression is significantly more than that of cucurbitacins E and D.

摘要

目的

葫芦素具有一系列抗癌功能。我们研究了从罗汉果（L.）A. Rich果实中纯化得到的葫芦素D、E和I对人胃癌细胞系AGS中一些凋亡和自噬基因的影响。

材料与方法

使用定量逆转录PCR（qRT-PCR），在分别用浓度为0.3、0.1和0.5μg/ml的葫芦素D、E和I处理AGS细胞24小时后，对Beclin-1、LC3、ATG5、ATG7和p62基因的表达进行定量。通过流式细胞术分析细胞周期和细胞死亡情况。

结果

纯化的葫芦素诱导AGS细胞出现亚G1期细胞周期阻滞和细胞死亡，并有效上调Beclin-1 mRNA，但对LC3、ATG5和p62 mRNA水平的影响非常低。同样，在用浓度为0.5μg/ml的葫芦素I处理后，p62 mRNA水平增加了约4.4倍。对葫芦素D、E和I对p62 mRNA表达影响的两两比较表明，葫芦素I的作用分别是葫芦素E和D的1.3倍和1.1倍；葫芦素D的作用是葫芦素E的1.2倍（P<0.05）。基因集富集分析表明，在自噬基因中，p62与胃癌具有重要的等级关系。

结论

从罗汉果果实中纯化得到的葫芦素D、E和I上调Beclin-1并诱导人胃癌细胞系AGS出现亚G1期细胞周期阻滞和细胞死亡。葫芦素I对p62 mRNA表达的影响明显大于葫芦素E和D。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/f5e4/5817168/e3281b64365c/IJBMS-21-253-g003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/f5e4/5817168/f2764531d343/IJBMS-21-253-g001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/f5e4/5817168/cd679b2ebcd9/IJBMS-21-253-g002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/f5e4/5817168/e3281b64365c/IJBMS-21-253-g003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/f5e4/5817168/f2764531d343/IJBMS-21-253-g001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/f5e4/5817168/cd679b2ebcd9/IJBMS-21-253-g002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/f5e4/5817168/e3281b64365c/IJBMS-21-253-g003.jpg

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