阿尔茨海默病小鼠模型中海马神经元的体内双光子钙成像

In Vivo Two-Photon Calcium Imaging of Hippocampal Neurons in Alzheimer Mouse Models.

作者信息

Busche Marc Aurel

机构信息

Department of Neurology, Massachusetts General Hospital, Harvard Medical School, MassGeneral Institute for Neurodegenerative Disease, Charlestown, MA, USA.

Department of Psychiatry and Psychotherapy, Technical University of Munich, Munich, Germany.

出版信息

Methods Mol Biol. 2018;1750:341-351. doi: 10.1007/978-1-4939-7704-8_23.

DOI:10.1007/978-1-4939-7704-8_23

PMID:29512084

Abstract

The use of in vivo two-photon microscopy in mouse models of Alzheimer's disease (AD) has propelled studies of disease mechanisms and treatments. For instance, this approach allowed for the first time to study in the intact brain the dynamics of individual amyloid plaques, and the effects of anti-amyloid therapies on plaque formation and growth. Moreover, by combining two-photon microscopy with fluorescent calcium indicators, an amyloid-dependent abnormal hyperactivity of cortical and hippocampal neurons was revealed as a primary neuronal impairment, which was not predicted from previous in vitro analyses. Here, a method for in vivo two-photon calcium imaging with single-cell and single-action potential accuracy in the hippocampus of Alzheimer mouse models is presented.

摘要

在阿尔茨海默病（AD）小鼠模型中使用体内双光子显微镜推动了疾病机制和治疗方法的研究。例如，这种方法首次使人们能够在完整大脑中研究单个淀粉样斑块的动态变化，以及抗淀粉样蛋白疗法对斑块形成和生长的影响。此外，通过将双光子显微镜与荧光钙指示剂相结合，发现皮质和海马神经元的淀粉样蛋白依赖性异常多动是一种主要的神经元损伤，这在以前的体外分析中并未预测到。在此，我们介绍一种在阿尔茨海默病小鼠模型海马中进行具有单细胞和单动作电位精度的体内双光子钙成像的方法。